Determination of olanzapine by spectrophotometry using permanganate

Rajendraprasad, N. and Basavaiah, K. (2009) Determination of olanzapine by spectrophotometry using permanganate. Brazilian Journal of Pharmaceutical Sciences, 45 (3). pp. 539-550. ISSN 2175-9790

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Two new spectrophotometric methods using permanganate as the oxidimetric reagent for the determination of olanzapine (OLP) were developed and validated as per the current ICH guidelines. The methods involved the addition of known excess of permanganate to OLP in either acid or alkaline medium followed by the determination of unreacted permanganate at 550 nm (method A) or blush-green color of manganate at 610 nm (method B). The decrease in absorbance in method A or increase in absorbance in method B as a function of concentration of OLP was measured and related to OLP concentration. Under optimized conditions, Beer's law was obeyed over the ranges 2.0 to 20 and 1.0 to 10 mu g mL(-1) in method A and method B, respectively. The calculated molar absorptivity values were 1.34 x 10(4) and 2.54 x 104 l mol(-1) cm(-1) for method A and method B respectively, and the respective Sandell sensitivities were 0.0233 and 0.0123 mu g cm(-2). The LOD and LOQ for method A were calculated to be 0.37 and 1.13 mu g mL(-1) and the corresponding values for method B were 0.16 and 0.48 mu g mL(-1). Intermediate precision, expressed as RSD was in the range 0.51 to 2.66%, and accuracy, expressed as relative error ranged from 0.79 to 2.24%. The proposed methods were successfully applied to the assay of OLP in commercial tablets with mcan percentage revoveries of 102 +/- 1.59 % (method A) and 101 +/- 1.53% (method B). The accuracy and reliability of the methods were further confirmed by performing recovery tests via standard addition procedure.

Item Type: Article
Uncontrolled Keywords: Olanzapine/determination; Spectrophotometry; Permanganate; Pharmaceuticals
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Users 19 not found.
Date Deposited: 22 Aug 2019 06:39
Last Modified: 22 Aug 2019 06:39

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