Chitosan induced resistance to downy mildew in sunflower caused by Plasmopara halstedii

Nandeeshkumar, P. and Sudisha, J. and Ramachandra Kini, K. and Prakash, H. S. and Niranjana, S. R. and Shekar Shetty, H. (2008) Chitosan induced resistance to downy mildew in sunflower caused by Plasmopara halstedii. Physiological and Molecular Plant Pathology, 72 (4). 188 - 194. ISSN 1096-1178

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Official URL: https://doi.org/10.1016/j.pmpp.2008.09.001

Abstract

Induction of resistance to downy mildew caused by Plasmopara halstedii in sunflower was studied after treatment with chitosan. Treatment of sunflower seeds with 5 chitosan resulted in decreased disease severity and offered 46 and 52 protection under greenhouse and field conditions respectively. The induction of resistance to P. halstedii by chitosan was accompanied by the accumulation of various host defense-related enzymes in susceptible sunflower seedlings. Enhanced activation of catalase (CAT) and phenylalanine ammonia lyase (PAL) was observed at 6h and 9h post-inoculation respectively, while peroxidase (POX), polyphenol oxidase (PPO) and chitinase (CHI) levels were high at 12h post-inoculation in sunflower seedlings raised from seeds treated with chitosan. Northern hybridization analysis revealed increased levels of transcripts for five known defense response genes viz., Pr-1a, β-1,3-glucanase, chitinase, peroxidase and chalcone synthase in these seedlings. This enhanced and early activation of defense-related responses in the susceptible cultivar Morden treated with chitosan was comparable to that in the resistant cultivar. The results indicate that chitosan induced resistance against P. halstedii in sunflower is mediated through the enhanced expression of genes for defense-related proteins.

Item Type: Article
Uncontrolled Keywords: Elicitor, Defense-related proteins, Transcript accumulation, Induction of resistance, Sunflower, Downy mildew
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 20 Aug 2019 11:26
Last Modified: 20 Aug 2019 11:26
URI: http://eprints.uni-mysore.ac.in/id/eprint/6831

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