Micropropagation of an endangered Indian sandalwood (Santalum album L.)

Sanjaya and Muthan, Bagyalakshmi and Rathore, Thrilok Singh and Rai, V. Ravishankar (2006) Micropropagation of an endangered Indian sandalwood (Santalum album L.). Journal of Forest Research, 11 (3). pp. 203-209.

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Abstract

Santalum album is known as East Indian sandalwood. It is the most economically important tree harvested for heartwood oil, and India is among the chief exporters of sandalwood and its products. Multiple shoots were induced from nodal shoot segments derived from a 50- to 60-year-old candidate plus tree (CPT) on Murashige and Skoog (MS) medium supplemented with 0.53þinspaceµM α-naphthaleneacetic acid (NAA) and 11.09þinspaceµM 6-benzylaminopurine (BA). In vitro differentiated shoots were multiplied on MS medium with 0.53þinspaceµM NAA, 4.44þinspaceµM BA, and additives: 283.93þinspaceµM ascorbic acid, 118.10þinspaceµM citric acid, 104.04þinspaceµM cystine, 342.24þinspaceµM glutamine, and 10% (v/v) coconut milk. New shoots were harvested repeatedly for up to three subculture passages on fresh medium at 4-week intervals. Microshoots treated with 98.4þinspaceµM indole-3-butyric acid (IBA) for 48þinspaceh produced roots on growth-regulator-free, quarter-strength MS basal salts medium with vitamin B5 and 2% sucrose. In vitro root induction was achieved from microshoots pulsed with 1230þinspaceµM IBA for 30þinspacemin in soilrite rooting medium. The percentage of rooting in soilrite was higher than that for agar medium, and in vitro raised plants were established in the field and showed normal growth.

Item Type:	Article
Subjects:	B Life Science > Biotechnology
Depositing User:	LA manjunath user
Date Deposited:	19 Aug 2019 06:20
Last Modified:	30 Jun 2020 10:42
URI:	http://eprints.uni-mysore.ac.in/id/eprint/6748

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