Cysteine proteases from the asclepiadaceae plants latex exhibited thrombin and plasmin like activities

Shivaprasad, H. V. and Riyaz, M. and Venkatesh Kumar, R. and Dharmappa, K. K. and Tarannum, Shaista and Siddesha, J. M. and Rajesh, R. and Vishwanath, B. S. (2009) Cysteine proteases from the asclepiadaceae plants latex exhibited thrombin and plasmin like activities. Journal Of Thrombosis and Thyrombolysis, 28 (3). pp. 304-308. ISSN 1573-742X

Full text not available from this repository. (Request a copy)
Official URL: 10.1007/s11239-008-0290-2


In the present study we evaluated the presence of cysteine protease from the latex of four plants Asclepias curassavica L., Calotropis gigantea R.Br., Pergularia extensa R.Br. and Cynanchum puciflorum R.Br. belongs to the family Asclepiadaceae. Cysteine proteases from these plants latex exhibited both thrombin and plasmin like activities. Latex enzyme fraction in a concentration dependent manner induced the formation of clot in citrated blood plasma. Direct incubation of fibrinogen with latex enzyme fraction resulted in the formation of fibrin clot similar to thrombin enzyme. However prolonged incubation resulted in degradation of the formed fibrin clot suggesting plasmin like activity. Latex enzyme fraction preferentially hydrolyzed A alpha and B beta chains of fibrinogen to form fibrin clot. Latex enzyme fraction also hydrolyzed the subunits of fully cross linked fibrin efficiently, the order of hydrolysis was alpha-polymer > alpha-chains > beta-chain and gamma-gamma dimer. Cysteine proteases from all the four Asclepiadaceae plants latex exhibited similar action on fibrinogen and fibrin. This study scientifically validate the use of plant latex in stop bleeding and wound healing by traditional healers all over the world.

Item Type: Article
Uncontrolled Keywords: Asclepiadaceae; Cysteine proteases; Thrombin like enzymes; Plasmin like enzymes
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Biochemistry
Depositing User: Users 19 not found.
Date Deposited: 17 Aug 2019 11:42
Last Modified: 12 Mar 2020 08:07

Actions (login required)

View Item View Item