Chandramouli, K. H. and Thinunaiah, K. N. and Chandrashekar, A. and D'Souza, C. J. M. (2004) Interaction of 2-chloro-N-10-substituted phenoxazine with DNA and effect on DNA melting. NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 23 (10). pp. 1639-1656.
Full text not available from this repository. (Request a copy)Abstract
Five N-10-substituted phenoxazines having different R groups and -Cl substitution at C-2 were found to bind to calf-thymus DNA and plasmid DNA with high affinity as seen from by UV and CD spectroscopy. The effect of phenoxazines on DNA were studied using DNA-ethidium bromide complexes. Upon addition of phenoxazines, the ethidium bromide dissociated from the complex with DNA. The binding of phenoxazines to plasmid (P)UC18 reduced ethidium bromide binding as seen from the agarose gel electrophoresis. Butyl, and propyl substituted phenoxazines were able to release more ethidium bromide compared with that of acetyl substitution. Addition of phenoxazines also enhanced melting temperature of DNA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | phenoxazine; DNA; ethidium bromide; agarose gel electrophoresis; DNA melting temperature |
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Users 23 not found. |
| Date Deposited: | 20 Aug 2019 12:32 |
| Last Modified: | 20 Aug 2019 12:32 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/6649 |
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