In vitro propagation of oroxylum indicum Vent. a medicinally important forest tree

Dalal, N. V. and Rai, V. R. (2004) In vitro propagation of oroxylum indicum Vent. a medicinally important forest tree. JOURNAL OF FOREST RESEARCH, 9 (1). pp. 61-65. ISSN 1341-6979

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Official URL: DOI 10.1007/s10310-003-0055-x

Abstract

In vitro propagation of Oroxylum indicum Vent. was carried out using cotyledonary node explants. Among the different types of cytokinins used for culture establishment, 6-benzyladenine exhibited the best response with higher concentrations (8.87 mu M or above) for inducing multiple shoots. Inclusion of indole-3-acetic acid (2.85 mu M) into 6-benzyladenine-supplemented medium triggered a high frequency of response as well as a proliferation of shoots. The best medium for proliferation was Murashige-Skoog (MS) medium with 6-benzyladenine (8.87 mu M) and indole-3-acetic acid (2.85 mu M). However, incorporation of gibberellic acid (1.44 mu M) was mandatory to enhance shoot elongation. Repeated subculturing of cotyledonary node and in vitro developed nodal segments in MS medium with 6-benzyladenine (4.44 mu M) at 4-week intervals resulted in continuous mass multiplication of shoots without any evidence of decline. Root induction was best (91.6%) when MS strength was reduced to one-quarter and combined with a-naphthalene acetic acid (2.69 mu M) and indole-3-acetic acid (5.71 mu M), with a high survival rate (70-72%) of plantlets hardened in either soil rite or soil: sand: soil rite (1:1:2).

Item Type: Article
Uncontrolled Keywords: Oroxylum indicum; Mass propagation; Seedling material; Vulnerable; Ex situ conservation; Medicinal; Forest tree
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biochemistry
Depositing User: lpa venkatesh user
Date Deposited: 21 Aug 2019 09:48
Last Modified: 21 Aug 2019 09:48
URI: http://eprints.uni-mysore.ac.in/id/eprint/6618

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