First report of gummy stem blight caused by didymella bryoniae on muskmelon (Cucumis melo) in India

Sudisha, J. and Kumar, T. V. and Niranjana, S. R. and Shekar Shetty, H. (2004) First report of gummy stem blight caused by didymella bryoniae on muskmelon (Cucumis melo) in India. PLANT PATHOLOGY, 53 (4). p. 533.

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Official URL: Doi: 10.1111/j.1365-3059.2004.01031.x

Abstract

Since 1999, severe losses have been observed on seedlingsof muskmelon (Cucumis melo) in four commercial fieldsin the Doddaballapura, Nanjangud, Malvalli and Arasikereregions of Karnataka, India. Losses occurred during themonths of October and November. Disease symptomsincluded stem necrosis with an exudation of gummymaterial, angular water-soaked lesions on the leaves androtten fruits. The presence of black pycnidia was observedon the stems, leaves and fruits. A fungus was isolated fromdiseased stems, leaves and seeds, by plating surface-sterilizedplant tissues onto potato dextrose agar (PDA) medium.On PDA, the mycelium was olivaceous green and fewpycnidia were observed. White aerial mycelium was alsoproduced. Conidia were hyaline, cylindrical with roundedends, mostly nonseptate, but a few single-septate, and6–11 × 3 –5 μm in size. Based on the symptoms and themorphological characteristics, the fungus was identifiedas Didymella bryoniae(Keinath et al., 1995). The pathogencauses gummy stem blight of cucurbits on species ofCitrullus, Cucumisand Cucurbita(Keinath et al. 1995). Toconfirm pathogenicity of D. bryoniae, 30-day-old musk-melon plants were wounded using a syringe needle andinoculated at the collar region with a suspension of 12 ×105conidia mL−1in sterile distilled water. The plants werecovered with plastic bags for 2 days and kept at 23°C and90% relative humidity, with a 12 h photoperiod. Plantswere assessed 7 days after infection. Inoculated plantsproduced typical symptoms on stems, leaves and fruits

Item Type: Article
Subjects: B Life Science > Botany
Divisions: Department of > Botany
Depositing User: lpa venkatesh user
Date Deposited: 31 Aug 2019 07:43
Last Modified: 31 Aug 2019 07:43
URI: http://eprints.uni-mysore.ac.in/id/eprint/6562

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