Attenuation of quorum-sensing-dependent virulence factors and biofilm formation by medicinal plants against antibiotic resistant Pseudomonas aeruginosa

Sankar Ganesh, P. and Ravishankar Rai, V. (2018) Attenuation of quorum-sensing-dependent virulence factors and biofilm formation by medicinal plants against antibiotic resistant Pseudomonas aeruginosa. Journal of Traditional and Complementary Medicine, 8 (1). 170 - 177. ISSN 2225-4110

[img] Text (Full Text)
Attenuation of quorum-sensing-dependent virulence.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy
Official URL: https://doi.org/10.1016/j.jtcme.2017.05.008

Abstract

Pseudomonas aeruginosa use small signaling molecules such as acyl homoserine lactones (AHLs), which play an important role in release virulence factors and toxin for further establishment of host infection. Thus, involving with the QS system would provide alternative ways of preventing the pathogenicity. In the present study, totally six medicinal plants (Terminalia bellerica, Celastrus paniculatus, Kingiodendron pinnatum, Schleichera oleosa, Melastoma malabathricum, Garcinia gummi-gutta) were screened for anti-QS activity using biomonitor strain of Chromobacterium violaceum CV12472. The primary screening of antimicrobial activity of all the plant extracts have inhibited the growth of tested bacterial species. Of these at the sub-minimum inhibitory concentration the methanol extract of T. bellerica (0.0625–0.5 mg/ml) has significantly inhibited violacein production (20.07–66.22) in C. violaceum (CV12472). Consequently, the extract of T. bellerica has reduced the production of pyocyanin, exopolysaccharide and biofilm formation in P. aeruginosa strains. Fluorescence and scanning electron microscopy analysis confirmed the reduction of biofilm formation in P. aeruginosa strains when treated with T. bellerica. GC–MS analysis showed the active compounds inhibited the production of virulence factors of P. aeruginosa. The results suggest the possible use of this T. bellerica as an anti-QS and anti-biofilm agent to control Pseudomonas infection. Interference of QS provides an important means for the inhibition of bacterial virulence and thus aids in treatment strategies.

Item Type: Article
Uncontrolled Keywords: Meningitis, Acyl homoserine lactone, Violacein, Pyocyanin, Biofilm
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: manjula User
Date Deposited: 13 Aug 2019 11:30
Last Modified: 13 Aug 2019 11:30
URI: http://eprints.uni-mysore.ac.in/id/eprint/6336

Actions (login required)

View Item View Item