Biochemical defence responses of resistant and susceptible rice genotypes against blast pathogen magnaporthe oryzae

Chandrakanth, R. and Narasimha Murthy, K. and Devaki, N. S. (2018) Biochemical defence responses of resistant and susceptible rice genotypes against blast pathogen magnaporthe oryzae. Archives of Phytopathology and Plant Protection, 51 (15-16). pp. 852-878. ISSN 1477-2906

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Rice blast is the leading fungal disease which is caused by Magnaporthe oryzae that contributes for the significant decline in the rice yield throughout the globe. There is a need for the understanding of biochemical changes in rice plant during blast infection for the development of novel disease control strategies. In the present study, we isolated M. oryzae from the local paddy fields and the fungal isolates (VCF and PON) were identified by ITS-PCR using genomic DNA samples. Further, we inoculated resistant (BR2655 and TUNGA) and susceptible (INTAN and HR12) rice cultivars with PON and VCF isolates. PON isolate showed relatively high virulence compared to VCF and standard MTCC fungal strains. Therefore, we evaluated the effect of PON on the total protein content and plant defence-related key enzymes (peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, β-glucosidase, chitinase and lipoxygenase) activities between 24- and 120-hour post-inoculation (hpi). The results demonstrated the decrease in total protein content in all the inoculated cultivars. In addition, we observed the variation in the activity of peroxidase, polyphenol oxidase, β-glucosidase, chitinase and lipoxygenase at different time points in all the tested rice plants compared to respective controls. However, no significant difference was observed in the phenylalanine ammonia lyase activity relative to its control. Taken together, this study emphasizes on the variation in the activities of plant defence enzymes in different plant cultivars against the tested fungal pathogen and also implementation of defence enzymes as biochemical markers for resistant breeding.

Item Type: Article
Subjects: B Life Science > Molecular Biology
Divisions: Yuvaraj college > Molecular biology
Depositing User: Manjula P Library Assistant
Date Deposited: 08 Aug 2019 10:53
Last Modified: 08 Aug 2019 10:53

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