Anticancer activity of metal nanoparticles and their peptide conjugates against human colon adenorectal carcinoma cells

Jamuna Bai, A. and Ravishankar Rai, V. and Umashankara, M. (2018) Anticancer activity of metal nanoparticles and their peptide conjugates against human colon adenorectal carcinoma cells. Artificial Cells, Nanomedicine, and Biotechnology, 46 (7). pp. 1444-1451. ISSN 2169-1401

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Official URL: https://doi.org/10.1080/21691401.2017.1373655

Abstract

In the present study, nanoparticles of gold, iron oxide and zinc oxide (ZnO) were studied for cytotoxicity in the colorectal cancer cell HT 29. The metallic nanoparticles in the range of <50 and <100 nm were screened for anticancer activity by MTT assay. The nanoparticles were tested at concentrations ranging from 0.5 to 50 µg/ml. Zinc oxide exhibited significant anti-cancer activity in comparison to other nanoparticles. It had an IC50 value of 17.12 µg/ml. The mechanism of action was studied by fluorescence microscopy with acridine orange, propidium iodide and DAPI staining techniques. The ROS production of ZnO nanoparticles was determined by DCFH-DA. The ZnO nanoparticles were conjugated with novel hydrophobic peptides and evaluated for anticancer activity. It was observed that the nanoparticles peptide complex showed better cytotoxicity than either peptide or nanoparticle alone. Thus, the ZnO nanoparticles tested in our study has anticancer activity against colon cancer cells. It can also be conjugated with peptides and used for targeting cancer cells with higher efficacy.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: manjula User
Date Deposited: 08 Aug 2019 10:13
Last Modified: 08 Aug 2019 10:13
URI: http://eprints.uni-mysore.ac.in/id/eprint/6245

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