Detection and quantification of fumonisins from Fusarium verticillioides in maize grown in southern India

Chandra Nayaka, S. and Udaya Shankar, A. C. and Niranjana, S. R. and Wulff, Ednar G. and Mortensen, C. N. and Prakash, H. S. (2009) Detection and quantification of fumonisins from Fusarium verticillioides in maize grown in southern India. World Journal of Microbiology and Biotechnology, 26 (1). pp. 71-78. ISSN 1573-0972

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Abstract

Fumonisins are a group of fungal toxins, occurring worldwide in maize infected mainly by Fusariumverticillioides. This paper describes the level of fumonisins in maize seed samples and the ability of F. verticillioides strains isolated from maize seeds grown in India to produce fumonisins. Forty-three seed samples intended to be used for consumption were collected from different regions of Karnataka and Andhra Pradesh. The samples were subjected to the agar plate method for the detection of F. verticillioides. Identification of F. verticillioides was done based on morphological characters and further confirmed by polymerase chain reaction. The majority of the samples were infected by F. verticillioides and infection percentage in the individual samples ranged from 5 to 51%. Twenty-three out of 35 (65%) strains were positive for fumonisin production in high performance liquid chromatography (HPLC) and competitive direct-enzyme linked immuno sorbent assay (CD-ELISA). Fumonisin level in seed samples ranged from 200 to 1,722 μg/g using CD-ELISA. HPLC could differentiate FB1 and FB2 toxins; out of 35 strains, 14 (40%) showed both FB1 and FB2 production. These findings indicate that there may be a risk of human exposure to fumonisins through the consumption of F. verticillioides infected corn-based foods in India.

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Biotechnology
Depositing User:	LA manjunath user
Date Deposited:	29 Jul 2019 06:28
Last Modified:	29 Jul 2019 06:28
URI:	http://eprints.uni-mysore.ac.in/id/eprint/6161

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