Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark

Tarannum, Shaista and Mohamed, Riyaz and Vishwanath, B. S. (2012) Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark. Natural Product Research, 26 (18). pp. 1708-1711. ISSN 1478-6427

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Abstract

This study was carried out to investigate the anti-fertility and anti-venom activities of the extract of the stem bark of Butea monosperma by inhibiting hyaluronidase, which is a spreading factor and plays a role in fertilisation. Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with IC50 values 12.00 ± 0.45, 49.40 ± 1.58 µg and 125.42 ± 2.82 µg mL–1, respectively. In a zymogram assay, the extract showed differential inhibition towards hyaluronidase isoform preferentially with low-molecular weight isoforms. The V. russelli snake venom-induced hemorrhage was significantly reduced at 1:05 ratio of venom-to-extract in mouse. The high antioxidant activity and total phenolic content in the ethanolic extract strongly correlated with the hyaluronidase inhibition. The above results justify the traditional use of the stem bark of B. monosperma as a contraceptive and a strong antidote to snake venom.

Item Type:	Article
Additional Information:	PMID: 22007959
Uncontrolled Keywords:	Butea monosperma , hyaluronidase, anti-fertility, anti-snake venom, haemorrhage
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	25 Jul 2019 10:26
Last Modified:	25 Jul 2019 10:26
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5568

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