Biochemical characterization of Fusarium oxysporum f. sp cubense isolates from India

Bhuvanendra, K. H. and Udaya, S. A. C. and Chandra, N. S. and Ramachandra, K. K. and Shetty, H. S. and Prakash, H. S. (2010) Biochemical characterization of Fusarium oxysporum f. sp cubense isolates from India. African journal of biotechnology, 9 (4). pp. 523-530. ISSN 1684-5315

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The Fusarium wilt caused by Fusarium oxyspoum f. sp. cubense (Foc) is a major biotic constraint for banana production. The characteristics of F. oxyspoum f. sp. cubense isolates were investigated using electrophoretic studies of isozyme and whole-cell protein. The morphological characteristics of the isolates were very similar to each other. All the Foc isolates were pathogenic to banana cultivar 'Nanjangud Rasabale' but they did not induce any disease symptoms on cultivar 'Cavendish'. F. oxyspoum (Isolate 6) did not induce wilt symptoms on either 'Nanjangud' or 'Cavendish' cultivar. Isozyme banding patterns showed 46 scoreable markers and cluster analysis with UPGMA using genetic distance showed that the isolates belonged to three main groups. Group 1 contained isolates 1, 2, 4, 5, 7 and isolate 3 and 6 were placed in group 2 and 3. Results indicated that the estimated intra-specific variation may be more pronounced with isozyme analysis than with protein markers. The level of isozyme variability detected within F. oxysporum f. sp. cubense suggested that it is reliable, efficient and effective in determining genetic relationships among Foc isolates.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: lpa manjunath user
Date Deposited: 25 Jul 2019 10:18
Last Modified: 25 Jul 2019 10:18

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