Induction of apoptosis in mcf-7 cells by methanolic extract of clitoria ternatea L.

Priyanka, S. and Balaji, K. S. and Chandrashekara, K. T. and Rangappa, K. S. and Jayarama, S. (2015) Induction of apoptosis in mcf-7 cells by methanolic extract of clitoria ternatea L. International Journal of Applied Biology and Pharmaceutical Technology, 6 (4). pp. 80-86. ISSN 0976-4550

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Abstract

Aim: Investigation of apoptosis induction by methanolic extract ofClitoria ternatea L. flower against multiple cancer cell lines. Main Methods: In the present study cytotoxic activity of Clitoriaternatea L flower was determined using MTT cell viability assay. Theinduction of cell death/apoptosis was evaluated by light microscopy, DNAfragmentation and caspase-3 enzyme activation. Key Findings: The methanolic extract from C. ternatea (MECT) showedcytotoxic activity against several cancer cell lines. The most potent activityexhibited by MECT was against MCF-7 breast carcinoma cells with anIC50 value of 27.2 ± 2.6 µg/mL. Light microscopic evaluation clearlyindicated the apoptotic morphology of MECT treated cells. Treatment of MCF-7cells with various MECT concentrations resulted in growth inhibition andinduction of apoptosis as indicated by DNA fragmentation and caspase-3 enzymeactivation. Significance: The current report strongly suggests the pro-apoptoticproperties of C. ternatea flowers. Our findings demonstrate that C.ternatea phyto-constituents may have beneficial applications in the fieldof anti-cancer drug discovery.

Item Type: Article
Uncontrolled Keywords: Clitoria Ternatea and Apoptosis and MTT Assay and DNA fragmentation and Caspase-3.
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Shrirekha N
Date Deposited: 24 Jul 2019 05:37
Last Modified: 24 Jul 2019 05:38
URI: http://eprints.uni-mysore.ac.in/id/eprint/5495

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