Evaluation of separation parameters for selected organophosphorus fungicides of forensic importance by RP-HPTLC

Nagaraju, P. M. and Sanganalmath, P. U. and Kemparaju, K. and Mohan, B. M. (2012) Evaluation of separation parameters for selected organophosphorus fungicides of forensic importance by RP-HPTLC. Acta Chromatographica, 24 (2). pp. 253-262. ISSN 1233-2356

Text (Full Text) Evaluation of Separation Parameters for Selected.pdf - Published Version Restricted to Registered users only Download (142kB) | Request a copy

Abstract

Studies on the evaluation of separation efficacies are of primary interest to forensic analysts for newly available pesticides. In the present work, a method is described for the separation evaluation of three selected organophosphorus fungicides (OPFs) ditalimfos (D), edifenfos (E), and tolclofos-methyl (TM) by reversed-phase high-performance thin-layer chromatography (RP-HPTLC). The method is based on the separation of three OPFs on silica gel 60 RP-18WF254 plates with varying mobile phase compositions (10:0–6:4) of methanol-water. The relationship between the mobile phase composition and retention parameters (RF and RM) as well as separation parameters (ΔRF, RS, α, RFα) was analyzed. The three OPFs are completely separated from each other with ΔRF ≥ 0.04, except for the pair of compounds E-TM in the mobile phase composition 10:0, 7:3, 6.5:3.5, and 6:4. Under the chromatographic conditions used, TM was adsorbed the strongest, followed by E and D, which is indicated by the higher RM values obtained for TM. Peak resolution (RS) values greater than 1.5 were obtained over the entire range of mobile phase composition, except for the pair of compounds E-TM. The UV apex of maximum absorption was 200 nm for all the three OPFs as measured by multiwave-length scan in the UV range, and higher values of peak area and peak height were obtained at the same wavelength. The present study clearly indicates that the system of RPHPTLC with mobile phase methanol-water in a volume composition 8:2 (υ/υ) provides the optimum conditions for the separation of the three OPFs. However, the complete separation of E from TM was not achieved using this system.

Item Type:	Article
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	20 Jul 2019 05:36
Last Modified:	20 Jul 2019 05:36
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5391

Actions (login required)

View Item