Induced systemic resistance by 2,4-diacetylphloroglucinol positive fluorescent Pseudomonas strains against rice sheath blight

Reshma, P. and Naik, M. K. and Aiyaz, M. and Niranjana, S. R. and Chennappa, G. and Shaikh, S. S. and Sayyed, R. Z. (2018) Induced systemic resistance by 2,4-diacetylphloroglucinol positive fluorescent Pseudomonas strains against rice sheath blight. Indian Journal of Experimental Biology, 56 (3). pp. 207-212. ISSN 0975-1009

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Abstract

Rice sheath blight arising due to lack of systemic resistance against causative agent Rhizoctonia solani has been a major economic threat to rice growers across the globe. This warrants induced systemic resistance (ISR) in plant to make them disease resistant. Application of antibiotic producing Pseudomonads that also secrete defense inducing enzymes is known to impart resistance to plants. Here, we report induction of the induced systemic resistance (ISR) by six strains of rhizosphere fluorescent Pseudomonas possessing 2,4-diacetyl phloroglucinol (2,4-DAPG) antibiotic genes against rice sheath blight pathogen Rhizoctonia solani. In the preliminary screening, isolate EP5 (endophytic Pseudomonas fluorescens) showed 76.5% inhibition against R. solani. EP5 treated rice grains showed the highest germination of 96.6%, mean root length of 15.3 cm, shoot length of 12.6 cm and vigour index of 2104.9. Paddy grains treated with EP5 (through seed treatment followed by root dipping) upon challenge inoculation with R. solani (Rs1) gave higher activity of peroxidase (PO) 2.50 change in absorbance at 470 nm/min/mg protein), polyphenol oxidases (PPO), 2.25 change absorbance at 420 nm/ min/mg protein, phenyl alanine ammonia lyase (PAL) and 28.5 nmol transcinnamic acid/hr/mg protein. 2,4-DAPG positive fluorescent Pseudomonas strains produced PO, PPO, PAL and 28.5 nmol transcinnamic that resulted in ISR against rice sheath blight pathogen R. solani.

Item Type:	Article
Subjects:	B Life Science > Botany
Divisions:	Department of > Botany
Depositing User:	Manjula P Library Assistant
Date Deposited:	19 Jul 2019 08:54
Last Modified:	15 Jul 2022 10:48
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5376

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