Sensitive liquid Chromatography--tandem mass spectrometry method for the determination of olanzapine in human urine

Anil Kumar, U. R. and Bhasakra, B. L. and Basavaiah, K. (2012) Sensitive liquid Chromatography--tandem mass spectrometry method for the determination of olanzapine in human urine. Arabian Journal for Science and Engineering, 37 (5). pp. 1381-1387. ISSN 1319-8025

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Official URL: https://doi.org/10.1007/s13369-012-0249-7

Abstract

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC--MS--MS) method was developed to determine olanzapine (OLZ) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C18 column (Symmetry 3.5 μm, 50 × 4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of ammonium acetate (pH 7.8)-acetonitrile (10:90, v/v). The method was linear over a concentration range of 1--100 ngml−1. The lower limit of quantitation was 1 ngml−1. The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was <11.5 %. The accuracy determined at three concentrations (8.0, 50.0 and 85.0 ngml−1 OLZ) was within ±1.21 % in terms of relative errors.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: MUL SWAPNA user
Date Deposited: 18 Jul 2019 10:22
Last Modified: 18 Jul 2019 10:22
URI: http://eprints.uni-mysore.ac.in/id/eprint/5354

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