Sensitive and selective methods for the determination of Cyproheptadine in tablets using N-Bromosuccinimide and two dyes

Raghu, M. S. and Basavaiah, K. (2012) Sensitive and selective methods for the determination of Cyproheptadine in tablets using N-Bromosuccinimide and two dyes. Chemical Industry & Chemical Engineering Quarterly, 18 (3). pp. 449-458. ISSN 2217-7434

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Abstract

One titrimetric and two spectrophotometric methods are described for the determination of cyproheptadine hydrochloride (CPH) in bulk drug and tablets employing N-bromosuccinimide (NBS) as a brominating agent and two dyes, erioglaucine (EG) and meta-cresol purple (MCP) as auxiliary reagents. In titrimetry, a measured excess of NBS is added to an acidified solution of CPH and the unreacted NBS is determined iodometrically. Spectrophotometry involves the addition of a known excess of NBS to CPH in acid medium followed by estimation of residual NBS by reacting with a fixed amount of either erioglaucin and measuring the absorbance at 630 nm (method A) or meta-cresol purple and measuring the absorbance at 540 nm (method B). The titrimetric procedure is applicable over the range of 1.5-15 mg of CPH, and the reaction stoichiometry is found to be 1:2 (CPH: NBS). The spectrophotometric methods are applicable over the ranges of 0.1-2.0 mu g mL(-1) (method A) and 0.4-12 mu g mL(-1) (method B). The molar absorptivities are calculated to be 1.4x10(5) and 2.2x10(4) L mol(-1) cm(-1) for methods A and B, respectively, and the corresponding Sandell sensitivity values are 0.0023 and 0.0141 mu g cm(-2). The limits of detection are calculated and found to be 0.03 and 0.24 mu g mL(-1) for methods A and B, respectively with corresponding limits of quantification 0.09 and 0.71. The methods were applied to the assay of CPH in tablets, and the results were compared statistically with those of a reference method.

Item Type:	Article
Uncontrolled Keywords:	Cyproheptadine; Titrimetry; Spectrophotometry; N-bromosuccinimide; Pharmaceuticals
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	18 Jul 2019 10:05
Last Modified:	18 Jul 2019 10:05
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5351

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