Quantification of antioxidants in medicinal plants and foodstuffs using Ce(IV) with indigo carmine as Chromogenic Probe

Nagaraja, P. and Suma, A. and Aradhana, N. and Shivakumar, A. and Avinash, K. and Krishna, H. (2012) Quantification of antioxidants in medicinal plants and foodstuffs using Ce(IV) with indigo carmine as Chromogenic Probe. Food Analytical Methods, 5 (4). pp. 909-919. ISSN 1936-976X

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Official URL: https://doi.org/10.1007/s12161-011-9328-8

Abstract

A simple, novel, sensitive and diversely applicable antioxidant assay method has been developed for the determination of total antioxidant capacity of some foodstuffs and medicinal plants. The method is based on the oxidation of antioxidants by a known amount of Ce(IV) sulphate under slightly acidic medium and subsequently allowing the unreacted Ce(IV) to react with a known amount of Indigo Carmine dye followed by measuring the unreacted blue-coloured Indigo Carmine dye solution at λmaxþinspace=þinspace610 nm at room temperature. The antioxidant power was evaluated in terms of trolox equivalent antioxidant capacities and compared with those of ferric-reducing antioxidant power (FRAP--1.10 phenanthroline), Cu(II) reduction assay using bathocuproinedisulfonic acid disodim salt and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid methods. A good correlation (R2þinspace=þinspace0.926) was found with the classical spectrophotometric FRAP assay. The proposed method is reproducible and successfully applied to assess the total antioxidant capacities in foodstuffs, vegetables and medicinal plants. The obtained results were compared with reference methods. Interference from other substances was also studied.

Item Type: Article
Uncontrolled Keywords: Antioxidant, Spectrophotometry, Ce(IV), Indigo Carmine dye, Trolox equivalent antioxidant capacities (TEAC)
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: MUL SWAPNA user
Date Deposited: 18 Jul 2019 06:06
Last Modified: 18 Jul 2019 06:06
URI: http://eprints.uni-mysore.ac.in/id/eprint/5336

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