Molecular detection of endophytic, myrothecium spp. by its-sequencing technique

Haghighi, M. T. and Shahdoust, E. (2015) Molecular detection of endophytic, myrothecium spp. by its-sequencing technique. International Journal of Research Studies in Biosciences, 3 (1). pp. 60-66. ISSN 2349-0365

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Abstract

Endophytic fungi are microorganisms with immense production secondary metabolites having biological activity features, which exist within plants without any hazard for their living. Ten isolates of endophytic Myrothecium verrucaria species, recovered from bark and twigs of Garcinia spp. in order to detection and identification of this fungus plant, and to analyze their genome fingerprints with help of ITSsequencing. ITS region is one of the most famous nuclear DNA sequences for genetic inference at the generic and genus levels in fungus. Hence, the target regions of rDNA, ITS 1, ITS 2 regions and 5.8S gene were amplified using primers ITS 1 and ITS 4. In this research, the phylogenetic evolutionary relationship of the desired endophyte were evaluated from the sequences of the ITS region. The amplification and subsequent sequencing of highly conserved ITS region showed genetic similarity among 10 isolates of Myrothecium spp (M), and based on the polymorphisms of the ITS region, the 10 isolates gave a length of ~550 bp amplicon

Item Type: Article
Uncontrolled Keywords: Phylogenetic Relationship and Its Sequencing and Endophytes and Myrothecium Verrucaria and Garcinia and Polymorphism
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Shrirekha N
Date Deposited: 17 Jul 2019 05:28
Last Modified: 17 Jul 2019 05:28
URI: http://eprints.uni-mysore.ac.in/id/eprint/5282

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