Dibenzazepin hydrochloride as a new spectrophotometric reagent for determination of hydrogen peroxide in plant extracts

Nagaraja, P. and Prakash, J. S. and Asha, S. C. and Bhaskara, B. L. and Anil Kumar, S. (2012) Dibenzazepin hydrochloride as a new spectrophotometric reagent for determination of hydrogen peroxide in plant extracts. Environmental Monitoring and Assessment, 184 (10). pp. 5983-5988. ISSN 1573-2959

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Abstract

A rapid, simple, accurate, and sensitive visible spectrophotometric method for the determination of trace amounts of hydrogen peroxide in acidic buffer medium is reported. The proposed method is based on the oxidative coupling of Ampyrone with dibenzazepin hydrochloride by hydrogen peroxide in the buffer medium of pH 4.0 which is catalyzed by ferrous iron. The blue-colored product formed with maximum absorption at 620 nm was found to be stable for 2 h. Beer's law is obeyed for hydrogen peroxide concentration in the range of 0.03--0.42 μg ml−1. The optimum reaction conditions and other important optical parameters are reported. The molar absorptive and Sandell's sensitivity are found to be 5.89þinspace×þinspace104 mol−1 cm−1 and 0.57 g/cm2, respectively. The interference due to diverse ions and complexing agents was studied. The method is successfully applied to the determination of hydrogen peroxide in green plants satisfactorily.

Item Type:	Article
Uncontrolled Keywords:	Hydrogen peroxide, Dibenzazepin hydrochloride, Ampyrone, Visible spectrophotometry, Oxidative coupling, Plant extracts
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	16 Jul 2019 09:08
Last Modified:	16 Jul 2019 09:08
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5261

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