Silymarin potentiates the anti-inflammatory effects of Celecoxib on chemically induced osteoarthritis in rats

Ashkavand, Z. and Malekinejad, H. and Amniattalab, A. and Rezaei-Golmisheh, A. and Vishwanath, B. S. (2012) Silymarin potentiates the anti-inflammatory effects of Celecoxib on chemically induced osteoarthritis in rats. Phytomedicine, 19 (13). 1200 - 1205. ISSN 1618-095X

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Silymarin (SMN) is used as an antioxidant complex to attenuate the pro-oxidant effects of toxic agents. This study was carried out to investigate the effect of SMN, Celecoxib (CLX) individually and in combination on monoiodoacetate (MIA)-induced osteoarthritis (OA) in rat. Forty adult Wistar rats were assigned to control and test groups. Animals in the test group following OA induction were subdivided into 4 subgroups according to the treatment profile: OA+; received saline normal (5ml/kg, b.w.), OA+CLX+; received CLX (100mg/kg, orally), OA+SMN+, received SMN (50mg/kg, orally), and OA+CLX+SMN+, received both CLX and SMN. The animals received test compounds by gastric gavage for 14 consecutive days. Animals in the OA+ group showed a significant (p<0.01) increase in serum and synovial levels of IL-1β, while both test compounds reduced the IL-1β level. Both CLX and SMN lowered the OA-increased level of malondialdehyde by 77 and 79 and nitric oxide by 73 and 76, respectively, in the synovial tissue. Special safranin O (SO) histopathological staining revealed that CLX and SMN improved the MIA-induced destruction and fibrillation in cartilage surface. CLX and SMN regulated the MIA-up regulated IL-1β at mRNA level. The combination therapy resulted in an additive effect between CLX and SMN in biochemical, histopathological and molecular assays. These findings suggest that SMN exerts anti-inflammatory effect and also potentiates the anti-inflammatory effect of CLX on MIA-induced OA. The anti-inflammatory property of SMN may attribute to its antioxidant capacity, which affects the proinflammatory mediators at translational and transcriptional level.

Item Type: Article
Uncontrolled Keywords: Silymarin, Antioxidant, Interleukin-1β, Osteoarthritis, Proinflammatory mediators
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: C Swapna Library Assistant
Date Deposited: 16 Jul 2019 05:43
Last Modified: 16 Jul 2019 05:43

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