Antibacterial activity of 3,3′,4′-Trihydroxyflavone from Justicia wynaadensis against diabetic wound and urinary tract infection

Dsouza, Dorin and Lakshmidevi, N. (2018) Antibacterial activity of 3,3′,4′-Trihydroxyflavone from Justicia wynaadensis against diabetic wound and urinary tract infection. Brazilian Journal of Microbiology, 49 (1). 152 - 161. ISSN 1678-4405

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Official URL: https://doi.org/10.1016/j.bjm.2017.05.002

Abstract

The present investigation was designed to study the effect of an active compound isolated from Justicia wynaadensis against multi drug resistant organisms (MDRO's) associated with diabetic patients. The drug resistant pathogens implicated in wound and urinary tract infection of diabetic patients were isolated and identified by molecular sequencing. Solvent–solvent fractionation of crude methanol extract produced hexane, chloroform, ethyl acetate and methanol–water fraction, among which chloroform fraction was found to be potent when compared with other three fractions. Further, chloroform fraction was subjected to preparatory HPLC (High-Performance Liquid Chromatography), that produced four sub-fractions; chloroform HPLC fraction 1 (CHF1) through CHF4. Among the sub-fractions, CHF1 inhibited the pathogens effectively in comparison to other three sub-fractions. The purity of CHF1 was found to be >95. Therefore, CHF1 was further characterized by NMR and FTIR analysis and based on the structure elucidated, the compound was found to be 3,3′,4′-Trihydroxyflavone. The effective dose of this bioactive compound ranged from 32μg/mL to 1.2mg/mL. Thus, the present study shows that 3,3′,4′-Trihydroxyflavone isolated from J. wynaadensis is an interesting biopharmaceutical agent and could be considered as a source of antimicrobial agent for the treatment of various infections and used as a template molecule for future drug development.

Item Type: Article
Uncontrolled Keywords: 3,3′,4′-Trihydroxyflavone, , Antibacterial activity, UTI, Wound
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: manjula User
Date Deposited: 15 Jul 2019 07:46
Last Modified: 15 Jul 2019 07:46
URI: http://eprints.uni-mysore.ac.in/id/eprint/5189

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