A simple and sensitive spectrophotometric method for the determination of trace amounts of nitrite in environmental and biological samples using 4-amino-5-hydroxynaphthalene-2,7-disulphonic acid monosodium salt

Nagaraja, Padmarajaiah and Al-Tayar, Naef Ghllab S. and Shivakumar, Anantharaman and Shrestha, Ashwine K. and Gowda, Avinash K. (2010) A simple and sensitive spectrophotometric method for the determination of trace amounts of nitrite in environmental and biological samples using 4-amino-5-hydroxynaphthalene-2,7-disulphonic acid monosodium salt. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 75 (5). 1411 - 1416. ISSN 1873-3557

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Abstract

A very simple, sensitive, fairly selective and rapid spectrophotometric method for the determination of trace amounts of nitrite has been described. This method is based on the diazotized intramolecular coupling of electrophilic diazonium cation with the phenolic group of 4-amino-5-hydroxynaphthalene-2,7-disulphonic acid monosodium salt (AHNDMS) in a phosphate buffer solution of pH 7.5. The cyclic product has a purple color with maximum absorbance at 560nm and is stable for 6h. Optimum reaction conditions and other important analytical parameters for the maximum color development were established. Beer's law was found to obey for nitrite in the concentration range of 0.1–1.6μgml−1 with molar absorptivity of 2.6×104lmol−1cm−1 and Sandell's sensitivity of 0.0075μgml−1. The effect of interfering ions on the determination is described. The recommended method was applied for the determination of nitrite in different water, soil and human saliva samples. The performance of the recommended method was evaluated in terms of Student's t-test and variance ratio F-test, which indicated the significance of proposed method over the reference method.

Item Type: Article
Uncontrolled Keywords: Nitrite determination, Spectrophotometry, 4-Amino-5-hydroxynapthalene-2,7-disulphonic, acid monosodium salt, Nitrite in environmental samples, Nitrite in biological samples
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: lpa manjunath user
Date Deposited: 12 Jul 2019 10:42
Last Modified: 12 Jul 2019 10:42
URI: http://eprints.uni-mysore.ac.in/id/eprint/5155

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