Swetha Kumar, B. and Naveen Kumar, S. K. and Katkar, Gajanan D. and Girish, K. S. and Kemparaju, K. (2018) Inhibition of echis carinatus venom by DNA, a promising therapeutic molecule for snakebite management. Biochimica et Biophysica Acta (BBA) - General Subjects, 1862 (5). 1115 - 1125. ISSN 0006-3002
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Background E. carinatus bite is a serious threat to South-Asian countries including India, as it causes the highest number of deaths and debilitating sustained tissue necrosis at the bite site. One of our previous studies has demonstrated the strong interaction between DNA and E. carinatus venom. Therefore, in this study, the effect of DNA on E. carinatus venom has been examined. Methods Here we show that calf thymus DNA interact strongly with E. carinatus venom and inhibits its enzymatic and pharmacological activities such as proteolytic, hemolytic, hyaluronidase, L-amino acid oxidase, NETosis, hemorrhage, pro-coagulant, and lethality. Further, using immunoblots and immunofluorescence, the study demonstrates the inhibition of proteolytic cleavage of several surface receptors on PMNs, PBMCs, and platelets by the DNA. Conclusions This study for the first time explored the efficient inhibition of enzymatic, pharmacological and lethal properties of E. carinatus venom by the naked DNA and demonstrates the possible therapeutic application of it during snakebite management. General significance This study identifies naked DNA as an effective defense weapon that has got the therapeutic potential to inhibit the detrimental effects of E. carinatus bite.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | . venom, DNA, Protease activity, Collagen receptors, Inflammatory cells |
| Subjects: | C Chemical Science > Chemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Manjula P Library Assistant |
| Date Deposited: | 10 Jul 2019 07:04 |
| Last Modified: | 19 Dec 2020 05:08 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/5052 |
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