Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66

Shastry, R. P. and Dolan, Stephen K and Abdelhamid, Yassmin and Rai, V. Ravishankar and Welch, Martin (2018) Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66. FEMS Microbiology Letters, 365 (9). pp. 1-7. ISSN 1574-6968

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Abstract

The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from CS66 that was 96% identical to the well-characterised AHL-lactonase AiiA from Bacillus thuringiensis, but only 30% was identical to AHL-lactonases from other Gram-negative species. This confirms that close AiiA homologs can be found in both Gram-positive and Gram-negative bacteria. Purified AiiE exhibited potent AHL-lactonase activity against a broad range of AHLs. Furthermore, aiiE was able to reduce the production of secreted plant cell wall-degrading hydrolytic enzymes when expressed in trans in the economically important plant pathogen, Pectobacterium atrosepticum. Our results indicate the presence of a novel AHL-lactonase in Enterobacter sp. CS66 with significant potential as a biocontrol agent.

Item Type:	Article
Subjects:	B Life Science > Microbiology
Divisions:	Department of > Microbiology
Depositing User:	Manjula P Library Assistant
Date Deposited:	09 Jul 2019 11:43
Last Modified:	21 Jun 2022 07:19
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5024

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