Characterization of an extracellular Chitosanase from the Soil Bacterium Bacillus Cereusch12

Niveditha, Prakash and Shubha, Gopal (2017) Characterization of an extracellular Chitosanase from the Soil Bacterium Bacillus Cereusch12. IIOAB Journal, 8 (1). pp. 1-6. ISSN 0976-3104

Text (Full Text) CHARACTERIZATIONOF AN EXTRACELLULAR.pdf - Published Version Restricted to Registered users only Download (361kB) | Request a copy

Abstract

An extracellular chitosanase producing bacterium was isolated from soil samples in Mysuru, Karnataka, India and identified asBacillus cereus CH12 based on morphological, biochemical and 16S rRNA gene sequence. Presence of the chitosanase gene was detectedby partial gene sequencing. The chitosanase showed an activity of 11.16 U/ml after three days of incubation with 90% deacetylated chitosan. The enzyme was purified by gel filtration and its molecular weight was 30 kDa. Optimum temperature of the enzyme was 40 0C and optimum pH was 6.0. The enzyme was stable at a pH range of 5.0-8.0 and up to a temperature of 50 0C. The enzyme showed high specificity towards carboxymethyl cellulose (CM-cellulose). Analysis of the hydrolytic product of the enzyme showed the presence of chitooligosaccharides of a degree of polymerization of 3-5. Bacillus cereus CH12chitosanase can be useful in the degradation of cellulose and chitosan containing biomass and in the production of chitooligosaccharides

Item Type:	Article
Subjects:	B Life Science > Microbiology
Divisions:	Department of > Microbiology
Depositing User:	C Swapna Library Assistant
Date Deposited:	09 Jul 2019 10:11
Last Modified:	09 Jul 2019 10:11
URI:	http://eprints.uni-mysore.ac.in/id/eprint/5011

Actions (login required)

View Item