Comparative characterisation of Russell's viper (Daboia/Vipera russelli) venoms from different regions of the Indian peninsula

Prasad, N. B. and Uma, B. and Bhatt, Subramanya K. G. and Gowda, T. Veerabasappa (1999) Comparative characterisation of Russell's viper (Daboia/Vipera russelli) venoms from different regions of the Indian peninsula. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1428 (2-3). pp. 121-136.

[img] Text (Full Text )
1-s2.0-S0304416599000537-main.pdf - Published Version
Restricted to Registered users only

Download (859Kb) | Request a copy
Official URL: http://dx.doi.org/10.1016/S0304-4165(99)00053-7

Abstract

Russell's viper (Daboia/Vipera russelli) venom from different regions of India was subjected to chromatographic, electrophoretic, biochemical and immunological analysis. The elution profiles from ion-exchange chromatography and protein banding pattern from SDS-PAGE showed a significant variation in the constituents of venoms. The acidic proteins are found to be predominant in the venoms of eastern and western regions while basic proteins are the major contributors of the northern and southern regional venoms. The major variation of phospholipases A(2) in the venom samples of India may be described as: southern regional venom is rich in basic, toxic PLA(2) while this activity showed a dramatic decrease as one moves towards west, north and eastern regions of India. In addition, the caseinolytic, TAME-hydrolytic, anticoagulant, oedema-inducing and haemorrhagic activities of the venoms have also varied from one region to another. The muscle specimens of mice injected with venoms of different regions showed variable change in the muscle fibre damage and cell morphology. The eastern regional venom is most lethal among all the venoms. The lethal potencies for four regional venoms vary as: eastern > western > southern > northern. The polyclonal antibodies prepared against the venom of southern region showed cross-reaction with the venoms of other regions, but the extent of cross-reaction and diffusion patterns are different. However, the polyclonal antibodies prepared against southern regional venom showed no protection against lethal toxicity of other regional venoms. (C) 1999 Elsevier Science B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: venom variation; phospholipase A(2); polyclonal antiserum; Daboial Vipera russelli
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Users 13 not found.
Date Deposited: 18 Apr 2013 10:52
Last Modified: 28 Oct 2015 06:05
URI: http://eprints.uni-mysore.ac.in/id/eprint/4903

Actions (login required)

View Item View Item