Sensitive and selective extractive spectrophotometric method for the Determination of hydroxyzine dihydrochloride in pharmaceuticals

Rajendraprasad, N. and Basavaiah, K. and Vinay, K. B. and Revanasiddappa, H. D. (2010) Sensitive and selective extractive spectrophotometric method for the Determination of hydroxyzine dihydrochloride in pharmaceuticals. Journal of the Mexican Chemical Society, 54 (4). pp. 233-239. ISSN 1870-249X

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Abstract

Hydroxyzine dihydrochloride (HDH), a piperazine H-1-receptor antagonist and antihistamine, is a rapid acting anxiolytic used principally as an anti-emetic. A sensitive, selective, and precise and accurate spectrophotometric method based on the formation of an ion-pair with orange II (ORG II) as ion-pair complexing agent was developed and validated for the determination of HDH in pharmaceuticals. The chloroform-extractable ion-pair complex exhibited an absorption maximum at 480 nm. Optimization of different experimental conditions is described. Beer's law is obeyed in the range of 1.5-15 mu g mL(-1) with an apparent molar absorptivity value of 2.07 x 10(4) L mol(-1) cm(-1) and Sandell's sensitivity value of 0.0216 mu g cm(-2). The limit of detection (LOD) and limit of quantification (LOQ) are 0.14 and 0.41 mu g mL(-1), respectively. A Job's plot of absorbance versus molar ratio of HDH to ORG II indicated (1:2) stoichiometric ratio. Within-and between-day relative standard deviations at three different concentration levels were < 3%. The developed method was successfully applied to commercial tablets. The results obtained were in good agreement with those obtained using the official method. No interference was encountered from co-formulated substances. Recoveries were 96-109 %.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: LA manjunath user
Date Deposited: 05 Jul 2019 11:13
Last Modified: 12 Mar 2020 07:40
URI: http://eprints.uni-mysore.ac.in/id/eprint/4791

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