Expression of β-hexosaminidase in the male reproductive system of the lizard, Eutropis carinata. (Reptilia, Squamata) (Schneider, 1801)

Medini, R. and Bhagya, M. and Ravindra, P. V. (2018) Expression of β-hexosaminidase in the male reproductive system of the lizard, Eutropis carinata. (Reptilia, Squamata) (Schneider, 1801). Cell and Tissue Research, 374 (2). pp. 413-421. ISSN 1432-0878

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Abstract

Beta-hexosaminidase (Hex) is the major lysosomal enzyme associated with the event of fertilization. In this study, we haveanalyzed the distribution of Hex in the testis and the epididymis of the lizard,Eutropis carinataby a polyclonal antibody ofβ-hexosaminidase isoform (Hex A). Presence of Hex in the epididymis was performed by Western blotting. The resultreveals that Hex A is present in the epididymal epithelium, lumen as well as spermatozoa. The anatomical distribution ofHex was studied by immunohistochemical localization. The study reveals that Hex is intensely stained in the epithelium ofanterior and middle regions of the epididymis, whereas, posterior epididymal epithelium shows moderate staining. Inaddition, seminiferous epithelium of the testis shows staining for Hex. But lumen of the testis did not show any reactionfor Hex. Further, immunohistochemical localization of Hex on the spermatozoa from the testis and different regions of theepididymis revealed that the Hex from the testis did not show any staining; the epididymal epithelium is moderately localizedin the spermatozoa of the anterior region and gradually increases in the intensity in the spermatozoa of the posterior region ofthe epididymis. This indicates that the Hex is released from the epididymal epithelium and binds to the spermatozoa, and inthe lumen, it gradually increases from anterior to the posteriorregion of the epididymis. The result also suggests that Hex Abound to the epididymal spermatozoa originates from the epididymis and not from the testis. The regional difference in theexpression of Hex in the epididymis of the lizard,E. carinata, indicates the possible site of secretion of this enzyme

Item Type:	Article
Subjects:	B Life Science > Zoology
Divisions:	Department of > Zoology
Depositing User:	Manjula P Library Assistant
Date Deposited:	04 Jul 2019 07:42
Last Modified:	04 Jul 2019 07:42
URI:	http://eprints.uni-mysore.ac.in/id/eprint/4594

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