Phylogenetic affiliation and determination of bioactive compounds of bacterial population associated with organs of mud crab, Scylla olivacea

Zote, Joanne and Passari, Ajit Kumar and Zothanpuia and Chandra Nayaka, S. and Senthil Kumar, Nachimuthu and AbdAllah, Elsayed Fathi and Hashem, Abeer and Alqarawi, Abdulaziz A. and Malik, Jahangir Ahmad and Singh, Bhim Pratap (2018) Phylogenetic affiliation and determination of bioactive compounds of bacterial population associated with organs of mud crab, Scylla olivacea. Saudi Journal of Biological Sciences, 25 (8). 1743 - 1754. ISSN 2213-7106

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Official URL: https://doi.org/10.1016/j.sjbs.2018.08.025

Abstract

Mud crab belongs to the genus Scylla is an economically valuable and preferred species for costal aquaculture in Asian countries, including India. In recent years, there has been a tremendous expansion of Scylla farming, which has led to increasing research on its habit and habitats. However, there has been no study undertaken to understand the role of the bacterial population associated with the different organs of the mud crab, Scylla olivacea. In total, 43 isolates were recovered from four selected parts of the crab (carapace, n = 18; abdomen n = 11; leg, n = 8; and hand, n = 6), and the 16S rRNA gene was used to identify the bacterial isolates. The antimicrobial potential along with the detection of modular polyketide synthase (PKSI), cytochrome P450 hydroxylase (CYP) and non-ribosomal peptide synthetase (NRPS) gene clusters were investigated to show a relationship among the biosynthetic genes with their useful aspects. Additionally, the potential three strains (BPSCRB12, BPSCRB14 and BPSCRB41), which showed significant antimicrobial activities, also showed the presence of twenty volatile compounds (VOCs) using GC–MS analysis. We conclude that the strain Aneurinibacillus aneurinilyticus BPSCRB41 could be source for the production of bioactive compounds.

Item Type: Article
Uncontrolled Keywords: , 16S rRNA gene, Modular polyketide synthase (PKSI), Cytochrome P450 hydroxylase(CYP), Non-ribosomal peptide synthetase (NRPS)
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 03 Jul 2019 11:20
Last Modified: 03 Jul 2019 11:20
URI: http://eprints.uni-mysore.ac.in/id/eprint/4573

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