Serodiagnosis of pearl millet resistance to downy mildew by quantitating cell wall P/HRGP using polyclonal antiserum Pab-P/HRGP

Deepak, Shantharaj and Shailasree, Sekhar and Sujeeth, Neerakkal and Kini, K. Ramachandra and Mithoefer, Axel and Shetty, H. Shekar (2008) Serodiagnosis of pearl millet resistance to downy mildew by quantitating cell wall P/HRGP using polyclonal antiserum Pab-P/HRGP. EUROPEAN JOURNAL OF PLANT PATHOLOGY, 121 (1). pp. 77-85.

[img] Text (Full Text)
10.pdf - Published Version
Restricted to Registered users only

Download (335Kb) | Request a copy
Official URL: http://dx.doi.org/10.1007/s10658-007-9246-1

Abstract

Proline/hydroxyproline-rich glycoprotein (P/HRGP) level in pearl millet genotypes resistant to downy mildew increase after inoculation with the oomycete pathogen Sclerospora graminicola. Using purified P/HRGPs from pearl millet cell walls, polyclonal antibodies (Pab-P/HRGP) were raised in rabbit. Based on this antiserum, an enzyme immunoassay was developed that displays a linearity detection range from 0.01 to 10 mu g P/HRGP. Western blot analysis, confirming the induction of three marker P/HRGPs in the infected resistant genotype, and immunocytochemical studies on P/HRGP localization either in epidermal peelings or in suspension-cultured cells demonstrated the specificity of the antiserum. Besides its characterization, Pab-P/HRGP was employed to screen various genotypes of pearl millet for fast, sensitive and specific detection of induced P/HRGPs upon infections. The results presented are discussed with presumed importance to downy mildew disease and the use of this new antiserum in pearl millet screening for disease resistance.

Item Type: Article
Uncontrolled Keywords: downy mildew; enzyme immunoassay; immunological quantification; proline/hydroxyproline rich-glycoproteins
Subjects: Agriculture Biological Sciences > Botany
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Botany

Agriculture Biological Sciences > Biotechnology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biotechnology
Divisions: PG Campuses > Manasagangotri, Mysore > Biotechnology
PG Campuses > Manasagangotri, Mysore > Botany
Depositing User: Users 8 not found.
Date Deposited: 15 Apr 2013 09:23
Last Modified: 13 Aug 2013 09:52
URI: http://eprints.uni-mysore.ac.in/id/eprint/4524

Actions (login required)

View Item View Item