Crocin prevents sesamol-induced oxidative stress and apoptosis in human platelets

Thushara, R. M. and Hemshekhar, M. and Paul, M. and Shanmuga Sundaram, M. and Shankar, R. L. and Kemparaju, K. and Girish, K. S. (2014) Crocin prevents sesamol-induced oxidative stress and apoptosis in human platelets. Journal of Thrombosis and Thrombolysis, 38 (3). pp. 321-330. ISSN 1573-742X

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Abstract

Recent studies have reported the platelet proapoptotic propensity of plant-derived molecules such as, resveratrol, thymoquinone, andrographolide and gossypol. Meanwhile, there were also reports of phytochemicals such as cinnamtannin B1, which shows antiapoptotic effect towards platelets. Platelets are mainly involved in hemostasis, thrombosis and wound healing. However, altered platelet functions can have serious pathological outcomes that include cardiovascular diseases. Platelets are sensitive to external and internal stimuli including therapeutic and dietary components. The anuclear platelets do undergo apoptosis via mitochondrial pathway. However, exaggerated rate of platelet apoptosis could lead to thrombocytopenia and other bleeding disorders. The present study deals with ameliorative efficacy of crocin on sesamol-induced platelet apoptosis. The antiapoptotic property of crocin and the proapoptotic tendency of sesamol in platelets were previously demonstrated. Therefore, it was interesting to see how these two compounds would interact and wield their effects on human platelets. Crocin effectively inhibited sesamol-induced oxidative stress on platelets, which was evidenced by the measurement of endogenously generated reactive oxygen species, particularly hydrogen peroxide, and changes in thiol levels. Further, crocin abrogated sesamol-induced biochemical events of apoptosis in platelets, which include intracellular calcium mobilization, changes in mitochondrial membrane integrity, cytochrome c release, caspase activity and phosphatidylserine externalization. Even though sesamol has proapoptotic effects on platelets, its anti-platelet activity cannot be neglected. Thus, the study proposes that sesamol could be supplemented with crocin, an approach that could not only abolish the toxic effects of sesamol on platelets, but also enhance the quality of treatment due to their synergistic action.

Item Type:	Article
Uncontrolled Keywords:	apoptosis, controlled study, human, human cell, unclassified drug, Phenols, cytotoxicity, Humans, drug effect, male, Male, female, Female, metabolism, priority journal, antioxidant, Antioxidants, enzyme activity, 1, cytology, hydrogen peroxide, article, phenol derivative, Apoptosis, reactive oxygen metabolite, oxidative stress, Oxidative Stress, drug potentiation, Blood Platelets, rotenone, thrombocyte, calcium cell level, drug activity, clinical trial, drug effects, cell protection, Carotenoids, crocin, carotenoid, plant medicinal product, calcimycin, caspase, cytochrome c, membrane depolarization, phosphatidylserine, mitochondrial membrane potential, 3 benzodioxole derivative, antiapoptotic activity, Benzodioxoles, calcium mobilization, cell interaction, protein secretion, sesamol
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	20 Aug 2019 10:16
Last Modified:	20 Aug 2019 10:16
URI:	http://eprints.uni-mysore.ac.in/id/eprint/4407

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