Structural and functional diversity of rhizobacteria associated with Rauwolfia spp. across the Western Ghat regions of Karnataka, India

Prasanna Kumar, S. P. and Hariprasad, P. and Brijesh Singh, S. and Gowtham, H. G. and Niranjana, S. R. (2014) Structural and functional diversity of rhizobacteria associated with Rauwolfia spp. across the Western Ghat regions of Karnataka, India. World Journal of Microbiology and Biotechnology, 30 (1). pp. 163-173. ISSN 1573-0972

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Abstract

The present study carried out with denaturing gradient gel electrophoresis of DNA extracted from rhizosphere soils of Rauwolfia spp. collected from Western Ghat (WG) regions of Karnataka indicated that Pseudomonas sp. was prevalently found followed by Methylobacterium sp., Bacillus sp. and uncultured bacteria. A total of 200 rhizobacteria were isolated from 58 rhizosphere soil samples comprising of 15 different bacterial genera. The Shannon Weaver diversity index (H�) and Simpson's diversity index (D) were found to be 2.57 and 0.91 for cultivable bacteria, respectively. The total species richness of cultivable rhizobacteria was high in Coorg district comprising 15 bacterial genera while in Mysore district, four bacterial genera were recorded. Rarefaction curve analysis also indicated the presence of higher species richness in samples of Shimoga and Coorg. All the rhizobacteria were screened for their multiple plant growth promotion and disease suppression traits. The results revealed that 70 % of the isolates colonized tomato roots, 42 % produced indole acetic acid, 55 % solubilized phosphorus, while 43, 22, 27, 19, 40, 15 and 44 % produced siderophore, salicylic acid, hydrogen cyanide, chitinase, phytase, cellulase and protease, respectively. Rhizobacterial isolates showing antagonistic activity against Fusarium oxysporum and Aspergillus flavus were 53 and 33 %, respectively. Plant growth promotion studies revealed that most of the isolates increased percent germination with significantly higher vigour index as compared to untreated control. Most predominant rhizobacteria found in the rhizospheres of Rauwolfia spp. of WG regions are potential PGPR which can serve as biofertilizers and biopesticides.

Item Type: Article
Uncontrolled Keywords: metabolism, Fusarium oxysporum, classification, genetics, microbiology, India, Aspergillus, Bacteriology, article, tomato, bacterium, Biodiversity, Bacteria, Soils, pH, Electrophoresis, Antagonism, plant development, Plant Development, Plants (botany), Aspergillus flavus, Plant growth promoting rhizobacteria, Lycopersicon esculentum, Bacteria (microorganisms), methodology, biodiversity, Rhizobiales, Acetic acid, Pseudomonas, rhizosphere, Rhizosphere, Bacillus (bacterium), Bacillus sp., Denaturing gradient gel electrophoreses (DGGE), denaturing gradient gel electrophoresis, Denaturing Gradient Gel Electrophoresis, Indoleacetic acid, Methylobacterium, Methylobacterium sp., Phosphate solubilization, phytohormone, Plant Growth Regulators, Polycyclic aromatic hydrocarbons, Pseudomonas sp., Rauvolfia, Rauwolfia, Rauwolfia spp, Soil Microbiology, uncultured bacterium
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 20 Aug 2019 06:10
Last Modified: 20 Aug 2019 06:10
URI: http://eprints.uni-mysore.ac.in/id/eprint/4396

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