Antidiabetic effects of Mukia maderaspatana and its phenolics: An in vitro study on gluconeogenesis and glucose uptake in rat tissues

Srilatha, B. R. and Ananda, S. (2014) Antidiabetic effects of Mukia maderaspatana and its phenolics: An in vitro study on gluconeogenesis and glucose uptake in rat tissues. Pharmaceutical Biology, 52 (5). pp. 597-602. ISSN 1744-5116

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Context: Traditional medicine is used by over 60% of the world's population for health care. Mukia maderaspatana (L.) M. Roem. (Cucurbitaceae) (Mukia) is extensively used in folklore medicine as an antidiabetic plant. It is rich in phenolics that contribute to its medicinal properties. Objective: Mukia extract and phenolics such as quercetin and phloroglucinol are investigated for their in vitro antidiabetic activity. Materials and methods: Quercetin, phloroglucinol, and methanol extract of the dried whole plant (0.25 and 0.5mg/ml) were studied for the inhibition of gluconeogenesis in rat liver slices and glucose uptake in isolated rat hemi-diaphragm (50 and 100μg/ml). Phenolics of Mukia were analyzed by HPLC. Results and discussion: Glucose (1.2mg/g/h) was synthesized from pyruvate and the synthesis was completely inhibited by insulin (1U/ml). Quercetin at 0.25 and 0.5mg/ml caused 65% and 89% inhibition (0.42mg/g/h and 0.13mg/g/h glucose). Addition of insulin did not increase inhibition. Phloroglucinol inhibited 100% glucose production with or without insulin. Mukia (0.25mg/ml) inhibited gluconeogenesis (0.65mg/g/h) by 45%, and with insulin, inhibition increased to 50% (0.59mg/g/h). At 0.5mg/ml, glucose production was stimulated by1.2-fold, but with insulin it was inhibited by 89% (0.13mg/g/h glucose). Mukia had no effect on glucose uptake, but potentiated the action of insulin mediated glucose uptake (152.82±13.30mg/dl/g/30min) compared with insulin control (112.41±9.14mg/dl/g/30min) (p\textless0.05). HPLC analysis revealed the presence of phenolics. Conclusion: Results provide scientific rationale for the use of Mukia in folk medicine as an antidiabetic nutraceutical.

Item Type: Article
Uncontrolled Keywords: controlled study, in vitro study, nonhuman, unclassified drug, Phenols, animal, Animals, chemistry, animal tissue, male, Male, rat, Rats, metabolism, glucose, plant extract, article, antidiabetic activity, antidiabetic agent, glucose transport, insulin, phenol derivative, high performance liquid chromatography, Hypoglycemic Agents, isolation and purification, Chromatography, drug effects, Glucose, Liver, quercetin, Quercetin, gluconeogenesis, Gluconeogenesis, pyruvic acid, liver, hemidiaphragm, Cucurbitaceae, diaphragm, Diaphragm, High Pressure Liquid, human insulin, In Vitro Techniques, liver slice, Mukia maderaspatana, Mukia maderaspatana extract, phloroglucinol, Phloroglucinol
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 20 Aug 2019 05:17
Last Modified: 20 Aug 2019 05:17

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