Rajesh, P. S. and Ravishankar Rai, V. (2014) Quorum quenching activity in cell-free lysate of endophytic bacteria isolated from Pterocarpus santalinus Linn., and its effect on quorum sensing regulated biofilm in Pseudomonas aeruginosa PAO1. Microbiological Research, 169 (7-8). pp. 561-569.
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Abstract
Quorum sensing mechanism allows the microorganisms to resist the antibiotic treatment by forming biofilms. Quorum quenching is one of the mechanisms to control the development of drug resistance in microbes. Endophyte bacteria are beneficial to plant growth as they support the immune system against the pathogen attack. The endophytic bacteria present in Pterocarpus santalinus were screened for the presence of N-acyl homoserine lactones (AHLs) degrading bacteria using biosensor strains and further confirmed by quantifying the violacein production. Cell-free lysate of endophytic bacteria, Bacillus firmus PT18 and Enterobacter asburiae PT39 exhibited potent AHL degrading ability by inhibiting about 80% violacein production in biosensor strain. Furthermore, when the cell-free lysate was applied to Pseudomonas aeruginosa PAO1 and PAO1-JP2 biofilm it resulted in significant (p\textless. 0.01) inhibition of biofilm formation. The biofilm inhibition was confirmed by visualization of biofilm slides under fluorescence microscopy, which showed decrease in total biomass formation in treated slides. Isolation and amplification of the gene (aiiA) indicated that the presence of AHL lactonase in cell-free lysate and sequence alignment indicated that AiiA contains a "HXHXDH" zinc-binding motif that is being conserved in several groups of metallohydrolases. Therefore, the study shows the potential of AHLs degradation by AHL lactonase present in cell-free lysate of isolated endophytic bacteria and inhibition of quorum sensing regulated biofilm formation in P. aeruginosa PAO1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | chemistry, metabolism, biofilm, genetics, enzyme activity, microbiology, physiology, Bacteriology, enzymology, amino acid sequence, Pterocarpus, Pterocarpus santalinus, Fluorescence microscopy, phylogeny, Phylogeny, pathogen, isolation and purification, Bacteria, Amino acids, Biofilms, Pseudomonas aeruginosa, bacterial protein, Bacterial Proteins, Enterobacter, quorum sensing, endophyte, Endophytes, Biosensors, Amino Acid Sequence, sequence alignment, Sequence Alignment, Bacteria (microorganisms), Molecular Sequence Data, microbial activity, growth rate, molecular genetics, Bacillus, Quorum Sensing, Carboxylic Ester Hydrolases, Enterobacter asburiae, Quorum quenching, plant community, Acyl-Butyrolactones, Bacillus (bacterium), Bacillus firmus, Biofilm inhibitions, carboxylesterase, gamma butyrolactone derivative, Lactonase, N-acyl homoserine lactonase, Quenching, Strain |
| Subjects: | B Life Science > Microbiology |
| Divisions: | Department of > Microbiology |
| Depositing User: | Arshiya Kousar Library Assistant |
| Date Deposited: | 22 Aug 2019 09:40 |
| Last Modified: | 22 Aug 2019 09:40 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/4388 |
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