Zearalenone induced toxicity in SHSY-5Y cells: The role of oxidative stress evidenced by N-acetyl cysteine

Venkataramana, M. and Chandra Nayaka, S. and Anand, T. and Rajesh, R. and Aiyaz, M. and Divakara, S. T. and Murali, H. S. and Prakash, H. S. and Lakshmana Rao, P. V. (2014) Zearalenone induced toxicity in SHSY-5Y cells: The role of oxidative stress evidenced by N-acetyl cysteine. Food and Chemical Toxicology, 65. pp. 335-342.

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Official URL: https://doi.org/10.1016/j.fct.2013.12.042

Abstract

Zearalenone (ZEN) is a mycotoxin from Fusarium species commonly found in many food commodities and are known to cause reproductive disorders, genotoxic and immunosuppressive effects. Although many studies have demonstrated the cytotoxic effects of ZEN, the mechanisms by which ZEN mediates its cytotoxic effects appear to differ according to cell type and route of exposure. Meantime, the available information on the neurotoxic effects of ZEN is very much limited. In the present study we evaluated the role of oxidative stress in ZEN mediated neurotoxicity in SH-SY5Y cells and investigated the possible underlying mechanism. ZEN induced ROS formation and elevated levels of MDA, loss of mitochondrial membrane potential (MMP) and increase in DNA damage in a dose dependent manner as assessed by COMET assay and agarose gel electrophoresis. However, there was no DNA damage by plasmid breakage assay at 6, 12 and 24. h time points. DAPI staining showed apoptotic nuclei at 12 and 24. h. Further, ZEN treated SH-SY5Y cells showed a marked suppressive effect on the neuronal gene expression. Use of an antioxidant N-acetylcysteine (NAC) reversed the toxin-induced generation of ROS and also attenuated loss of MMP. Collectively, these results suggest that ROS is the main upstream signal leading to increased ZEN mediated neurotoxicity in SH-SY5Y cells.

Item Type: Article
Uncontrolled Keywords: apoptosis, Article, cell viability, controlled study, human, human cell, Cell Line, Humans, Tumor, cell survival, antioxidant activity, gene expression, messenger RNA, reactive oxygen metabolite, Reactive Oxygen Species, lipid peroxidation, Mitochondrial, oxidative stress, Oxidative Stress, signal transduction, malonaldehyde, drug determination, tyrosine 3 monooxygenase, upregulation, DNA damage, brain derived neurotrophic factor, Zearalenone, zearalenone, neurotoxicity, DNA Primers, comet assay, Membrane Potential, mitochondrial membrane potential, AADC gene, acetylcysteine, Acetylcysteine, agar gel electrophoresis, aromatic levo amino acid decarboxylase, Base Sequence, BDNF gene, cytotoxicity test, double stranded DNA break, gene targeting, matrix metalloproteinase, Mycotoxin, NAC, neuroblastoma cell, Neurotoxicity, Real-Time Polymerase Chain Reaction, ROS, TH gene
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 30 Aug 2019 05:23
Last Modified: 30 Aug 2019 05:23
URI: http://eprints.uni-mysore.ac.in/id/eprint/4334

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