Spectrophotometric determination of folic acid in pharmaceutical preparations by coupling reactions with iminodibenzyl or 3-aminophenol or sodium molybdate-pyrocatechol

Nagaraja, P. and Vasantha, R. A. and Yathirajan, H. S. (2002) Spectrophotometric determination of folic acid in pharmaceutical preparations by coupling reactions with iminodibenzyl or 3-aminophenol or sodium molybdate-pyrocatechol. ANALYTICAL BIOCHEMISTRY, 307 (2). pp. 316-321.

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Official URL: http://dx.doi.org/10.1016/S0003-2697(02)00038-6

Abstract

Novel coupling reagents are used for the simple and sensitive spectrophotometric determination of folic acid either in pure form or in its pharmaceutical preparations. The methods are based on the probable diazotization of the p-aminobenzoylglutamic acid obtained after reductive clevage of folic acid, followed by either coupling with iminodibenzyl to give a violet product with lambda(max) Of 580 urn or coupling with 3-aminophenol to produce an orange yellow-colored product with lambda(max) of 460 nm. Sodium molybdate and pyrocatechol are used in the third method and the pale red-colored product formed has a lambda(max) of 490 nm. The methods are highly reproducible and have been applied to the determination of folic acid in tablets and the results compare favorably with the official method. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed methods. (C) 2002 ElsevierScience (USA). All rights reserved.

Item Type: Article
Uncontrolled Keywords: folic acid; spectrophotometry; iminodibenzyl; 3-aminophenol; pyrocatechol; sodium molybdate; pharmaceuticals
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Users 11 not found.
Date Deposited: 15 Apr 2013 07:07
Last Modified: 29 Aug 2013 11:24
URI: http://eprints.uni-mysore.ac.in/id/eprint/4290

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