Mohamed, R. and Tarannum, S. and Yariswamy, M. and Vivek, H. K. and Siddesha, J. M. and Angaswamy, N. and Vishwanath, B. S. (2014) Ascorbic acid 6-palmitate: A potent inhibitor of human and soybean lipoxygenase-dependent lipid peroxidation. Journal of Pharmacy and Pharmacology, 66 (6). pp. 769-778. ISSN 0022-3573
Full text not available from this repository. (Request a copy)Abstract
Objectives Lipoxygenases (LOX) are the key enzymes involved in the biosynthesis of leukotrienes and reactive oxygen species, which are implicated in pathophysiology of inflammatory disorders. This study was conducted to evaluate the inhibitory effect of water-soluble antioxidant ascorbic acid and its lipophilic derivative, ascorbic acid 6-palmitate (Vcpal) on polymorphonuclear lymphocyte 5-LOX and soybean 15-LOX (sLOX) in vitro. Methods LOX activity was determined by measuring the end products, 5-hydroperoxy eicosatetraenoic acid (5-HETE) and lipid hydroperoxides, by spectrophotometric and high performance liquid chromatography methods. The substrate-dependent enzyme kinetics and docking studies were carried out to understand the nature of inhibition. Key findings Vcpal potently inhibited 5-LOX when compared with its inhibitory effect on sLOX (IC50; 2.5 and 10.3μm respectively, P= 0.003). Further, Vcpal inhibited 5-LOX more strongly than the known synthetic drugs: phenidone and nordihydroguaiaretic acid (P= 0.0007). Enzyme kinetic studies demonstrated Vcpal as a non-competitive reversible inhibitor of 5-LOX. In-silico molecular docking revealed high MolDock and Rerank score for Vcpal than ascorbic acid, complementing in-vitro results. Conclusion Both in-vitro and docking studies demonstrated Vcpal but not ascorbic acid as a non-competitive inhibitor of 5-LOX- and sLOX-induced lipid peroxidation, suggesting a key role for lipophilic nature in bringing about inhibition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | controlled study, drug potency, human, human cell, in vitro study, unclassified drug, analogs and derivatives, drug structure, Humans, drug mechanism, molecular docking, Molecular Docking Simulation, neutrophil, Neutrophils, antioxidant, Antioxidants, enzyme activity, physiology, enzymology, article, high performance liquid chromatography, lipid peroxidation, Lipid Peroxidation, drug effects, ascorbic acid, spectrophotometry, IC 50, lipophilicity, Ascorbic Acid, lipoxygenase, arachidonate 5 lipoxygenase, Arachidonate 5-Lipoxygenase, lipoxygenase inhibitor, Lipoxygenase Inhibitors, 6-O-palmitoylascorbic acid, ascorbic acid 6 palmitate, enzyme substrate, nordihydroguaiaretic acid, phenidone, soybean, Soybeans |
| Subjects: | C Chemical Science > Biochemistry |
| Divisions: | Department of > Biochemistry |
| Depositing User: | Arshiya Kousar Library Assistant |
| Date Deposited: | 28 Jun 2019 10:35 |
| Last Modified: | 28 Jun 2019 10:35 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/3984 |
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