Circulatory autoantibodies against hyaluronic acid binding proteins: A novel serum biomarker

Nunna, V. and Banerjee, S. and Karuna Kumar, M. (2014) Circulatory autoantibodies against hyaluronic acid binding proteins: A novel serum biomarker. Asian Journal of Pharmaceutical and Clinical Research, 7 (2). pp. 199-203. ISSN 2455-3891

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Abstract

Objective: Circulating autoantibodies have been extensively investigated as possible markers for early diagnosis of cancer. The present study was carried out to investigate whether anti-HABPs autoantibodies could be classified as a serum biomarker for malignant tumors. Methods: An enzyme-linked immunosorbent assay, western blot was used to detect anti-HABPs autoantibodies in sera from 99 patients with various types of cancers and 50 healthy subjects matched by age and gender. Result: Our results clearly indicated that patients with cancer have significant higher circulating levels of anti-HABPs antibodies as compared to control subjects (\textless 0.001). Receiver operating characteristic plot test has exhibited 91.9% sensitivity and 76.3% specificity. Conclusion: anti-HABPs autoantibodies are promising biomarker for malignant tumors and could play a role in the development of multimarker assay for the early detection of cancer. Abbreviations: HABPs, hyaluronic acid binding proteins; ELISA, enzyme linked immune sorbent assay; AAbs, autoantibodies; BSA, bovine serum albumin; HA, hyaluronic acid; bHA, biotinlyted hyaluronic acid; ROC, receiver operating characteristics; AUC, area under curve; TAA, tumor associated antigen; ABTS, 2,2'azino bis (3ethylbenzothiazoline 6 sulphonic acid); MES buffer, 2 (N morpholino) ethanesulfonic acid; EDC,1 Ethyl 3 (3 dimethylamino propyl) carbodiimide; DMSO, dimethyl sulfoxide; PVDF, polyvinylidene fluoride; ECL, enhanced chemiluminescence.

Item Type:	Article
Uncontrolled Keywords:	controlled study, human, adult, male, female, major clinical study, age, Western blotting, article, gender, breast cancer, early diagnosis, enzyme linked immunosorbent assay, autoantibody, lung cancer, stomach cancer, colon cancer, sensitivity and specificity, uterine cervix cancer, biological marker, tumor marker, blood sampling, antibody blood level, antibody detection, cancer diagnosis, hyaluronic acid binding protein, rectum cancer
Subjects:	C Chemical Science > Biochemistry
Divisions:	Department of > Biochemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	01 Jul 2019 06:47
Last Modified:	10 Jul 2019 05:48
URI:	http://eprints.uni-mysore.ac.in/id/eprint/3965

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