New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf

Madhu, C. S. and Manukumar, H. M. G. and Basavaraju, P. (2014) New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf. Acta Scientiarum Polonorum Technologia Alimentaria, 13 (4). pp. 375-383. ISSN 1644-0730

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Abstract

Aim. This study describes antioxidant and anti-inflammatory properties of Sauropus androgynus leaf dialysed protein extract. Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. The main objective of the present study was to reveal the antioxidant and anti-inflammatory potentiality from S. androgynus leaf dialysed protein extract. Material and methods. The antioxidant and anti-inflammatory properties of S. androgynus studied using different models viz. hydroxyl radical scavenging, DPPH radical scavenging, reducing power assay, superoxide radical scavenging activity by alkaline DMSO and phosphomolybdenum antioxidant assays and in vitro antiinflammatory activity by inhibition of protein denaturation, membrane stabilisation test models carried out. Results. Antioxidant activity was estimated in dose dependent manner showed hydroxyl radical (55.62%), DPPH (50%), reducing power (0.286 Abs), alkaline DMSO (72.51%) and phosphomolybdenum (0.198 Abs) activity high at 50 μg/ml concentration compared to standard curcumin (62.31%), curcumin (56.61%), tannic acid (0.54 Abs), curcumin (75.38%) and vitamin E (0.15 Abs) respectively. In-vitro anti-inflammatory activity by hypotonic induced model showed maximum protection (74.17%) compared to standard Acetylsalcylic acid (86.88%) at 100 μg/ml concentration and also in protein denaturation model protected protein denaturation maximum (83.60%) compared to standard Diclofenac (86.82%) at 100 μg/ml concentration respectively. Conclusion. The antioxidant property usually studied related to the polyphenols and flavonoids present in the extract but present finding concluded that S. androgynus giving hint even potential proteins can also show responsible action effective against free radical mediated disease.

Item Type:	Article
Uncontrolled Keywords:	human, Phenols, chemistry, Humans, metabolism, plant protein, Plant Proteins, Anti-Inflammatory Agents, antiinflammatory agent, antioxidant, Antioxidants, 1, Anti-inflammatories, Anti-inflammatory activity, plant extract, Anti-oxidant activities, flavonoid, phenol derivative, Proteins, Diseases, plant leaf, protein denaturation, Plant Extracts, Free radicals, hydroxyl radical, Hydroxyl Radical, Plant Leaves, Assays, Disease control, Plants (botany), drug effects, 1-diphenyl-2-picrylhydrazyl, Biphenyl Compounds, biphenyl derivative, Picrates, picric acid, erythrocyte membrane, dimethyl sulfoxide, Dimethyl Sulfoxide, Oxygen, polyphenol, Flavonoids, angiosperm, Angiosperms, Antioxidant properties, Antioxidant therapies, Denaturation, Erythrocyte, Erythrocyte Membrane, Lysosomal, Neutrophil, Polyphenols, Protein Denaturation, Sauropus androgynus, vegetable, Vegetables
Subjects:	B Life Science > Biotechnology C Chemical Science > Biochemistry
Divisions:	Department of > Biotechnology Yuvaraj college > Bio-Chemistry
Depositing User:	Arshiya Kousar Library Assistant
Date Deposited:	01 Jul 2019 06:12
Last Modified:	01 Jul 2019 06:12
URI:	http://eprints.uni-mysore.ac.in/id/eprint/3960

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