Antioxidant and hepatoprotective effects of Solanum xanthocarpum leaf extracts against CCl4-induced liver injury in rats

Jalali Ghassam, B. and Ghaffari, H. and Prakash, H. S. and Kini, K. R. (2014) Antioxidant and hepatoprotective effects of Solanum xanthocarpum leaf extracts against CCl4-induced liver injury in rats. Pharmaceutical Biology, 52 (8). pp. 1060-1068. ISSN 1744-5116

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Context: Solanum xanthocarpum Schard. and Wendl. (Solanaceae) has been used in traditional Indian medicines for its antioxidant, anti-inflammatory, and antiasthmatic properties. Objective: The present study demonstrates the antioxidant and hepatoprotective effects of S. xanthocarpum. On the basis of in vitro antioxidant properties, the active fraction from column chromatography of the methanol extract of S. xanthocarpum leaves (SXAF) was chosen as the potent fraction and used for hepatoprotective studies in rats. Materials and methods: The antioxidant activity was evaluated by 2,2'-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and reducing power assays. Rats were pre-treated with 100 and 200mg/kg b.w. of SXAF for 14d with a single dose of CCl4 in the last day. Hepatoprotective properties were determined by serum biochemical enzymes, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), antioxidant enzymes (SOD, CAT, GSH, and GST), and histopathology studies. Results: SXAF exhibited significant antioxidant activity in scavenging free radicals with IC50 values of 11.72μg (DPPH) and 17.99μg (ABTS). Rats pre-treated with SXAF demonstrated significantly reduced levels of serum LDH (1.7-fold), ALP (1.6-fold), and AST (1.8-fold). Similarly, multiple dose SXAF administration at 200mg/kg b.w. demonstrated significantly enhanced levels of SOD (1.78±0.13), CAT (34.63±1.98), GST (231.64±14.28), and GSH (8.23±0.48) in liver homogenates. Histopathological examination showed lowered liver damage in SXAF-treated groups. Discussion and conclusion: These results demonstrate that SXAF possesses potent antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity.

Item Type: Article
Uncontrolled Keywords: animal experiment, animal model, column chromatography, controlled study, in vitro study, in vivo study, nonhuman, unclassified drug, animal, Animals, animal tissue, male, Male, rat, Rats, Wistar, Wistar rat, histopathology, antioxidant, antioxidant activity, Antioxidants, catalase, superoxide dismutase, drug efficacy, liver protective agent, plant extract, article, alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, glutathione, lipid peroxidation, plant leaf, isolation and purification, methanol, Plant Extracts, Plant Leaves, lactate dehydrogenase, treatment outcome, glutathione transferase, ABTS radical scavenging assay, ascorbic acid, DPPH radical scavenging assay, IC 50, liver protection, Solanum, Carbon Tetrachloride, Drug-Induced Liver Injury, liver injury, silymarin, Treatment Outcome, carbon tetrachloride, reducing power assay, antioxidant assay, Solanum virginianum, Solanum xanthocarpum extract
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 26 Jun 2019 06:01
Last Modified: 26 Jun 2019 06:01

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