Hepatoprotective and antioxidant activity of N-Trisaccharide in different experimental rats

Kavishankar, G. B. and Moree, S. S. and Lakshmidevi, N. (2014) Hepatoprotective and antioxidant activity of N-Trisaccharide in different experimental rats. Phytomedicine, 21 (8-9). pp. 1026-1031.

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Official URL: https://doi.org/10.1016/j.phymed.2014.04.033


Objectives To investigate the hepatoprotective, antioxidant and antihyperlipidemic effect of N-Trisaccharide isolated from Cucumis prophetarum (L.) on different experimental rats. Methods N-Trisaccharide (25 and 50 mg/kg.b.w), silymarin (25 mg/kg) and glibenclamide (25 mg/kg) was orally administered once daily for 28 days and toxicity evaluation studies were carried out. Liver damage was assessed by determining DNA damage, serum enzyme activities and hepatic histopathology of carbon tetrachloride (CCl4) induced hepatic injury in rats. Enzymatic and non enzymatic antioxidant levels in liver and kidney were determined and biochemical parameters such as, serum lipid profile, renal function markers were estimated in type 2 diabetic rats. Results DNA fragmentation analysis revealed the protective effect of N-Trisaccharide on liver DNA damage. Histopathological studies indicated that CCl4-induced liver injury was less severe in N-Trisaccharide (25 and 50 mg/kg) treated group. Given at the above doses conferred significant protection against the hepatotoxic actions of CCl4 in rats, reducing serum markers like SGOT, SGPT, ALP, creatinine and urea levels back to near normal (p < 0.05) compared to untreated rats. In diabetic rats, N-Trisaccharide treatment significantly reversed abnormal status of enzymatic and non-enzymatic antioxidants levels to near normal. Also, serum lipids such as TG, TC, LDL-C and VLDL-C levels were significantly (p < 0.05) reduced compared to diabetic untreated rats. Conclusion Present study results confirm that N-Trisaccharide possesses significant antihyperlipidemic, antioxidant and hepatoprotective properties.

Item Type: Article
Uncontrolled Keywords: animal experiment, animal model, controlled study, nonhuman, unclassified drug, animal, Animals, chemistry, animal tissue, male, Male, rat, Rats, Wistar, Wistar rat, chemically induced, Experimental, female, Female, histopathology, metabolism, pathology, priority journal, antioxidant, antioxidant activity, Antioxidants, glucose, glucose blood level, liver protective agent, plant extract, article, alanine aminotransferase, alanine aminotransferase blood level, alkaline phosphatase, alkaline phosphatase blood level, aspartate aminotransferase, aspartate aminotransferase blood level, glutathione, lipid peroxidation, urea, urea blood level, Diabetes Mellitus, glibenclamide, Glyburide, isolation and purification, lipid blood level, low density lipoprotein cholesterol, Plant Extracts, Lipid Peroxidation, Animal, disease model, Disease Models, non insulin dependent diabetes mellitus, glucose metabolism, drug effects, DNA damage, creatinine blood level, Administration, Liver, liver protection, DNA fragmentation, oral drug administration, Carbon Tetrachloride, Drug-Induced Liver Injury, liver injury, Rattus, silymarin, Silymarin, creatinine, hyperglycemia, Hypolipidemic Agents, liver, Oral, Cucumis, Cucumis prophetarum, trisaccharide, Trisaccharides, antilipemic agent, carbon tetrachloride, detoxification, DNA Fragmentation, kidney, Kidney, kidney function, n trisaccharide, very low density lipoprotein cholesterol
Subjects: B Life Science > Microbiology
C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Department of > Microbiology
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 26 Jun 2019 05:22
Last Modified: 26 Jun 2019 05:22
URI: http://eprints.uni-mysore.ac.in/id/eprint/3696

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