Anti-diabetic effect of a novel N-Trisaccharide isolated from Cucumis prophetarum on streptozotocin-nicotinamide induced type 2 diabetic rats

Kavishankar, G. B. and Lakshmidevi, N. (2014) Anti-diabetic effect of a novel N-Trisaccharide isolated from Cucumis prophetarum on streptozotocin-nicotinamide induced type 2 diabetic rats. Phytomedicine, 21 (5). pp. 624-630. ISSN 0944-7113

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Official URL: https://doi.org/10.1016/j.phymed.2013.12.002

Abstract

Ethnopharmacological relevance Cucumis prophetarum (L.) is used in traditional Indian medicine for the treatment of inflammation related problems. Aim of the study The present investigation was designed to study the effect of N-Trisaccharide (a new compound isolated from the fruit of C. prophetarum (L.)) on hyperglycemia in streptozotocin (STZ)-nicotinamide (NA) induced type 2 diabetic rats. Materials and methods Different doses of N-Trisaccharide (25 and 50 mg/kg b.w.) were administered once daily for 28 days to STZ-NA induced diabetic rats. Plasma insulin and glycogen levels were measured. The activities of hexokinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, glucose-6-phosphate dehydrogenase, glycogen synthase and glycogen phosphorylase were measured. Further, histological studies on pancreas were also carried out. Results The active compound at doses of 25 and 50 mg/kg b.w. given orally for 14 days showed 47.7% and 69.3% antihyperglycemic activity, respectively. Treatment at the same doses for 28 days provided complete protection against STZ-NA challenge (65 and 230 mg/kg b.w., respectively), intraperitoneally. N-Trisaccharide significantly (p 0.05) increased the plasma insulin and liver glycogen levels in diabetic rats. The altered enzyme activities of carbohydrate metabolism in the liver and kidney of the diabetic rats were significantly (p 0.05) improved. Additionally, N-Trisaccharide increased glycogen synthase and decreased glycogen phosphorylase activity in diabetic rats. Histological studies confirmed an increase in insulin level is due to stimulation of injured pancreatic β-cells. Conclusion The results of the study suggested that N-Trisaccharide possesses propitious effect on STZ-NA induced type 2 diabetes, indicating its usefulness in diabetes management.

Item Type: Article
Uncontrolled Keywords: animal experiment, animal model, controlled study, nonhuman, unclassified drug, animal, Animals, chemistry, drug screening, animal cell, male, Male, rat, Rats, Wistar, Wistar rat, chemically induced, Experimental, female, Female, metabolism, priority journal, enzyme activity, glucose, glucose blood level, enzymology, plant extract, article, antidiabetic activity, antidiabetic agent, insulin, glycogen, glycogen liver level, insulin blood level, medicinal plant, pancreas islet beta cell, Blood Glucose, Diabetes Mellitus, glibenclamide, Hypoglycemic Agents, isolation and purification, nicotinamide, Plant Extracts, streptozocin, non insulin dependent diabetes mellitus, Type 2, drug effects, Liver, fruit, Fruit, Drug Evaluation, Phytotherapy, Preclinical, Rattus, Plants, Medicinal, carbohydrate metabolism, glucose 6 phosphate dehydrogenase, Glycogen, liver, pancreas, histology, phytotherapy, Cucumis, Cucumis prophetarum, fructose bisphosphatase, glucose 6 phosphatase, glycogen phosphorylase, glycogen synthase, hexokinase, Pancreas, streptozotocin nicotinamide, trisaccharide, Trisaccharides
Subjects: B Life Science > Microbiology
C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Department of > Microbiology
Depositing User: Arshiya Kousar
Date Deposited: 27 Jun 2019 05:30
Last Modified: 27 Jun 2019 05:30
URI: http://eprints.uni-mysore.ac.in/id/eprint/3683

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