Spectrophotometric assay of creatinine in human serum sample

Avinash, K. and Nagaraja, P. and Shivakumar, A. and Krishna, H. and Nagaraja (2017) Spectrophotometric assay of creatinine in human serum sample. Arabian Journal of Chemistry, 10. S2018 - S2024. ISSN 1878-5352

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Official URL: https://doi.org/10.1016/j.arabjc.2013.07.030


A new spectrophotometric method for the analysis of creatinine concentration in human serum samples is developed. The method explores the oxidation of p-methylamino phenol sulfate (Metol) in the presence of copper sulfate and creatinine which yields an intense violet colored species with maximum absorbance at 530nm. The calibration graph of creatinine by fixed time assay ranged from 4.4 to 620μM. Recovery of creatinine in human serum samples varied from 101 to 106. Limit of detection and limit of quantification were 0.145μM and 0.487μM respectively. Sandell’s sensitivity was 0.112μgcm−2 and molar absorptivity was 0.101×104Lmol−1cm−1. Within day precision was 2.5–4.8 and day-to-day precision range was 3.2–7.8. The robustness and ruggedness of the method expressed in RSD values ranged from 0.78 to 2.12 and 1.32 to 3.46 respectively, suggesting that the developed method was rugged. This method provides good sensitivity and is comparable to standard Jaffe’s method with comparatively less interference from foreign substances.

Item Type: Article
Uncontrolled Keywords: Creatinine, Metol, Serum, Jaffe’s
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: C Swapna Library Assistant
Date Deposited: 24 Jun 2019 10:07
Last Modified: 24 Jun 2019 10:07
URI: http://eprints.uni-mysore.ac.in/id/eprint/3664

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