Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour

Kolapalli, Srinivasa Prasad and Nunna, Venatrao and Thomas, Anil and Karuna Kumar, M. and Banerjee, Shib Das and Boregowda, R. K. (2016) Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour. Cell Biochemistry and Function, 34 (4). pp. 217-225.

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Official URL: https://doi.org/10.1002/cbf.3179

Abstract

Tumour cells generate hyaluronan (HA) oligomers (O‐HA) by an autocrine mechanism to regulate their own behaviour through receptor interaction, necessitating analysis of HA sizes and its receptor expression in tumour progression. In this study for the first time, we identified specific size of HA in malignant ovarian tumour compared to benign tumour tissue. Therefore, we prepared the identified HA probes and conducted multiplex and monoplex ligand blot analysis and Immunohistochemistry to identify their receptor expression and distribution. Although, HA recognized CD44 as principle receptors despite of size, multiplex analysis showed multiple receptor expression with distribution at the tumour cell surface. Furthermore, the HA 6‐mer (major O‐HA of ovarian tumour) pull down of tumour tissue proteins showed 120 kDa protein along with CD44 with over expression in the malignant tumour. Upon depletion of CD44 protein HA 6‐mer showed a major 120 kDa protein with distribution at nuclear membrane, suggesting that this protein may play an important role in ovarian tumour progression. In summary, ovarian tumour cells of different grade showed heterogeneity in generation of HA oligomers and their interaction with specific receptors. Therefore, simultaneous analysis of O‐HA and their receptors expression could serve as a prognostic indicator during tumorigenesis. Copyright © 2016 John Wiley & Sons, Ltd.

Item Type: Article
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: manjula User
Date Deposited: 21 Jun 2019 05:50
Last Modified: 21 Jun 2019 05:50
URI: http://eprints.uni-mysore.ac.in/id/eprint/3578

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