Simple and sensitive spectrophotometric assay of isoniazid in pharmaceuticals using permanganate, methyl orange and indigo carmine as reagent

Basavaiah, K. and Swamy, N. and Chandrashekar, U. (2017) Simple and sensitive spectrophotometric assay of isoniazid in pharmaceuticals using permanganate, methyl orange and indigo carmine as reagent. Acta Poloniae Pharmaceutica - Drug Research, 74. pp. 1353-1364. ISSN 0001-6837

Text (Full Text) SIMPLE AND SENSITIVE SPECTROPHOTOMETRIC ASSAY OF ISONIAZID IN PHARMACEUTICALS USING PERMANGANATE, METHYL ORANGE AND INDIGO CARMINE AS REAGENT.pdf - Published Version Restricted to Registered users only Download (229kB) | Request a copy

Abstract

Two simple, sensitive and rapid spectrophotometric methods are described for the determination of isoniazid (INH) in pharmaceuticals. The methods are based on the oxidation of INH by a measured excess of KMnO4 in acid medium followed by the determination of residual oxidant by reacting with either methyl orange and measuring the absorbance at 520 nm (method A) or indigo carmine, when the absorbance was measured at 610 nm (method B). Experimental conditions such as KMnO4 and dye concentrations, acid medium and reaction time to yield better performance characteristics were carefully studied and optimized. Beerís law was obeyed in the concentration ranges, 0.25-5.0 and 0.25-4.0 µg/mL for method A and method B, respectively, and the corresponding molar absorptivity values were 2.5 x 10⁵ and 3.0 x 10⁵ L/M cm⁻¹. The limits of detection (LOD) and quantification (LOQ) were 0.08 and 0.23 µg/mL (method A) and 0.16 and 0.18 µg/mL (method B). Intra-day and inter-day accuracy expressed as relative error were better than 3% and the respective precision reported as relative standard deviation were also < 3%. Methods, when applied to tablets, yielded results which compared well with the label claim and also with those obtained by the reference method. No interference was observed from the tablet excipients.

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	C Swapna Library Assistant
Date Deposited:	20 Jun 2019 09:43
Last Modified:	20 Jun 2019 09:43
URI:	http://eprints.uni-mysore.ac.in/id/eprint/3541

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