Quercetin-3-O-rhamnoside from Euphorbia hirta protects against snake Venom induced toxicity

Gopi, Kadiyala and Anbarasu, K. and Renu, Kadali and Jayanthi, S. and Vishwanath, B. S. and Jayaraman, Gurunathan (2016) Quercetin-3-O-rhamnoside from Euphorbia hirta protects against snake Venom induced toxicity. Biochimica et Biophysica Acta (BBA) - General Subjects, 1860 (7). 1528 - 1540.

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Official URL: https://doi.org/10.1016/j.bbagen.2016.03.031

Abstract

Background The plant Euphorbia hirta is widely used against snake envenomations in rural areas and it was proved to be effective in animal models. Therefore, the scientific validation of its phytoconstituents for their antiophidian activity is aimed in the present study. Methods E. hirta extract was subjected to bioactivity guided fractionation and the fractions that inhibited different enzyme activities of Naja naja venom in vitro was structurally characterized using UV, FT-IR, LC–MS and NMR spectroscopy. Edema, hemorrhage and lethality inhibition activity of the compound were studied in mice model. In addition, molecular docking and molecular dynamic simulations were also performed in silico. Results The bioactive fraction was identified as Quercetin-3-O-α-rhamnoside (QR, 448.38 Da). In vitro experiments indicated that protease, phospholipase-A2, hemolytic activity and hemorrhage inducing activity of the venom were inhibited completely at a ratio of 1:20 (venom: QR) w/w. At the same concentration, the edema ratio was drastically reduced from 187 to 107. Significant inhibition (93) of hyaluronidase activity was also observed at a slightly higher concentration of QR (1:50). Further, in in vivo analysis, QR significantly prolonged the survival time of mice injected with snake venom. Conclusion For the first time Quercetin-3-O-α-rhamnoside, isolated from E. hirta, has been shown to exhibit anti-snake venom activity against Naja naja venom induced toxicity. General significance Exploring such multifunctional lead molecules with anti-venom activity would help in developing complementary medicine for snakebite treatments especially in rural areas where anti-snake venom is not readily available.

Item Type: Article
Uncontrolled Keywords: , Snake venom, , Quercetin-3-O-rhamnoside, PLA
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: manjula User
Date Deposited: 20 Jun 2019 09:34
Last Modified: 20 Jun 2019 09:34
URI: http://eprints.uni-mysore.ac.in/id/eprint/3540

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