Inhibition of Naja naja venom hyaluronidase by plant-derived bioactive components and polysaccharides

Girish, K. S. and Kemparaju, K. (2005) Inhibition of Naja naja venom hyaluronidase by plant-derived bioactive components and polysaccharides. BIOCHEMISTRY-MOSCOW, 70 (8). pp. 948-952.

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The inhibitory effect of several bioactive compounds on the activity of hyaluronidase enzyme purified from Naja naja venom was investigated in vitro. Compounds were found to inhibit the hyaluronidase activity dose dependently. Among glycosaminoglycans, heparin, heparan sulfate, and dermatan sulfate showed maximum inhibition compared to chondroitin sulfates. Different molecular forms of chitosan inhibit the enzyme, and inhibition appears to depend on the chain length. In addition, plant-derived bioactive compounds also inhibited the activity of hyaluronidase dose dependently. Among those tested, aristolochic acid, indomethacin, quercetin, curcumin, tannic acid, and flavone exhibited inhibition, with aristolochic acid and quercetin completely inhibiting the enzyme activity. It is concluded that the inhibitors of hyaluronidase could be used as potent first aid agents in snakebite therapy. Furthermore, these inhibitors not only reduce the local tissue damage but also retard the easy diffusion of systemic toxins and hence increase survival time.

Item Type: Article
Uncontrolled Keywords: hyaluronidase; inhibitors; spreading factor; Naja naia venom
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Users 9 not found.
Date Deposited: 09 Apr 2013 10:37
Last Modified: 08 Aug 2013 05:37

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