Brain aging, memory impairment and oxidative stress: A study in Drosophila melanogaster

Haddadi, M. and Jahromi, S. R. and Sagar, B. K. C. and Patil, R. K. and Shivanandappa, T. and Ramesh, S. R. (2014) Brain aging, memory impairment and oxidative stress: A study in Drosophila melanogaster. Behavioural Brain Research, 259. pp. 60-69. ISSN 0166-4328

Full text not available from this repository. (Request a copy)
Official URL:


Memory impairment during aging is believed to be a consequence of decline in neuronal function and increase in neurodegeneration. Accumulation of oxidative damage and reduction of antioxidant defense system play a key role in organismal aging and functional senescence. In our study, we examined the age-related memory impairment (AMI) in relation to oxidative stress using Drosophila model. We observed a decline in cognitive function in old flies with respect to both short-lived and consolidated forms of olfactory memory. Light and electron microscopy of mushroom bodies revealed a reduction in the number of synapses and discernible architectural defects in mitochondria. An increase in neuronal apoptosis in Kenyon cells was also evident in aged flies. Biochemical investigations revealed a comparable age-associated decrease in the activity of antioxidant enzymes such as catalase and superoxide dismutase as well as the GSH level, accompanied by an increase in the level of lipid peroxidation and generation of reactive oxygen species in the brain. There was no significant difference in the activity level of AChE and BChE enzymes between different age groups while immunohistochemical studies showed a significant decrease in the level of ChAT in 50-day-old flies. RNAi-mediated silencing of cat and sod1 genes caused severe memory impairment in 15-day-old flies, whereas, over-expression of cat gene could partially rescue the memory loss in the old flies. We demonstrated that a Drosophila long-lived strain, possessing enhanced activity of antioxidant enzymes and higher rate of resistance to oxidative stress, shows lower extent of AMI compared to normal lifespan strain. Present study provides evidence for involvement of oxidative stress in AMI in Drosophila.

Item Type: Article
Uncontrolled Keywords: apoptosis, controlled study, lifespan, nonhuman, Animals, animal cell, animal tissue, Time Factors, Gene Expression Regulation, immunohistochemistry, priority journal, aged, enzyme activity, catalase, Catalase, superoxide dismutase, Superoxide Dismutase, disease severity, article, acetylcholinesterase, cholinesterase, biochemistry, RNA interference, reactive oxygen metabolite, Reactive Oxygen Species, glutathione, lipid peroxidation, mitochondrion, oxidative stress, Oxidative Stress, gene, Lipid Peroxidation, microscopy, Brain, Drosophila melanogaster, synapse, Oxidative stress, Glutathione, gene silencing, Drosophila Proteins, Aging, nerve cell, Acetylcholine, Age-related memory impairment, brain aging, brain region, CAT gene, Choline, choline acetyltransferase, Choline O-Acetyltransferase, Classical, Classical olfactory conditioning, cognitive defect, Conditioning, electron microscopy, gene overexpression, Genetically Modified, memory disorder, Memory Disorders, Mushroom Bodies, mushroom body, nervous system parameters, olfactory memory, Smell, SOD1 gene, strain difference
Subjects: B Life Science > Zoology
Divisions: Department of > Zoology
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 24 Jun 2019 09:42
Last Modified: 24 Jun 2019 09:42

Actions (login required)

View Item View Item